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Title: Magnetoferritin enhances T₂ contrast in magnetic resonance imaging of macrophages
Authors: Kumar, Ambrish
Nandwana, Vikas
Ryoo, Soo-Ryoon
Ravishankar, Samyukta
Sharma, Bhargy
Pervushin, Konstantin
Dravid, Vinayak P.
Lim, Sierin
Keywords: Engineering::Bioengineering
Issue Date: 2021
Source: Kumar, A., Nandwana, V., Ryoo, S., Ravishankar, S., Sharma, B., Pervushin, K., Dravid, V. P. & Lim, S. (2021). Magnetoferritin enhances T₂ contrast in magnetic resonance imaging of macrophages. Materials Science and Engineering: C, 128, 112282-.
Project: M4081504.F40.706022
Journal: Materials Science and Engineering: C
Abstract: Imaging of immune cells has wide implications in understanding disease progression and staging. While optical imaging is limited in penetration depth due to light properties, magnetic resonance (MR) imaging provides a more powerful tool for the imaging of deep tissues where immune cells reside. Due to poor MR signal to noise ratio, tracking of such cells typically requires contrast agents. This report presents an in-depth physical characterization and application of archaeal magnetoferritin for MR imaging of macrophages - an important component of the innate immune system that is the first line of defense and first responder in acute inflammation. Magnetoferritin is synthesized by loading iron in apoferritin in anaerobic condition at 65 °C. The loading method results in one order of magnitude enhancement of r1 and r2 relaxivities compared to standard ferritin synthesized by aerobic loading of iron at room temperature. Detailed characterizations of the magnetoferritin revealed a crystalline core structure that is distinct from previously reported ones indicating magnetite form. The magnetite core is more stable in the presence of reducing agents and has higher peroxidase-like activities compared to the core in standard loading. Co-incubation of macrophage cells with magnetoferritin in-vitro shows significantly higher enhancement in T2-MRI contrast of the immune cells compared to standard ferritin.
ISSN: 0928-4931
DOI: 10.1016/j.msec.2021.112282
Schools: School of Chemical and Biomedical Engineering 
School of Biological Sciences 
Research Centres: NTU-Northwestern Institute for Nanomedicine 
Rights: © 2021 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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