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|Title:||Spatio-temporal in vivo imaging of ocular drug delivery systems using fiberoptic confocal laser microendoscopy||Authors:||Chaw, Su Yin
Wong, Tina Tzee Ling
Venkatraman, Subbu S.
|Keywords:||Engineering::Materials||Issue Date:||2021||Source:||Chaw, S. Y., Wong, T. T. L., Venkatraman, S. S. & Chacko, A. (2021). Spatio-temporal in vivo imaging of ocular drug delivery systems using fiberoptic confocal laser microendoscopy. Journal of Visualized Experiments (JoVE), 2021(175), e62685-. https://dx.doi.org/10.3791/62685||Project:||AG/CIV/GC70-C/NRF/2013/2
|Journal:||Journal of Visualized Experiments (JoVE)||Abstract:||Subconjunctival injection is an attractive route to administer ocular drugs due to easy trans-scleral access that bypasses anterior ocular barriers, such as the cornea and conjunctiva. While therapeutic effects and pharmacokinetics of the drugs upon subconjunctival injection have been described in some studies, very few assess the ocular distribution of drugs or drug delivery systems (DDS). The latter is critical for the optimization of intraocular DDS design and drug bioavailability to achieve the desired ocular localization and duration of action (e.g., acute versus. prolonged). This study establishes the use of fiberoptic confocal laser microendoscopy (CLM) to qualitatively study the ocular distribution of fluorescent liposomes in real-time in live mice after sub-conjunctival injection. Being designed for in vivo visual inspection of tissues at the microscopic level, this is also the first full description of the CLM imaging method to study spatio-temporal distribution of injectables in the eye after subconjunctival injection.||URI:||https://hdl.handle.net/10356/160751||ISSN:||1940-087X||DOI:||10.3791/62685||Schools:||School of Materials Science and Engineering||Organisations:||National University of Singapore||Rights:||© 2021 JoVE Journal of Visualized Experiments. All rights reserved. This paper was published in JoVE Journal of Visualized Experiments and is made available with permission of JoVE Journal of Visualized Experiments.||Fulltext Permission:||embargo_20231004||Fulltext Availability:||With Fulltext|
|Appears in Collections:||MSE Journal Articles|
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