Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/160751
Title: Spatio-temporal in vivo imaging of ocular drug delivery systems using fiberoptic confocal laser microendoscopy
Authors: Chaw, Su Yin
Wong, Tina Tzee Ling
Venkatraman, Subbu S.
Chacko, Ann-Marie
Keywords: Engineering::Materials
Issue Date: 2021
Source: Chaw, S. Y., Wong, T. T. L., Venkatraman, S. S. & Chacko, A. (2021). Spatio-temporal in vivo imaging of ocular drug delivery systems using fiberoptic confocal laser microendoscopy. Journal of Visualized Experiments (JoVE), 2021(175), e62685-. https://dx.doi.org/10.3791/62685
Project: AG/CIV/GC70-C/NRF/2013/2 
H18/01/a0/018 
Journal: Journal of Visualized Experiments (JoVE) 
Abstract: Subconjunctival injection is an attractive route to administer ocular drugs due to easy trans-scleral access that bypasses anterior ocular barriers, such as the cornea and conjunctiva. While therapeutic effects and pharmacokinetics of the drugs upon subconjunctival injection have been described in some studies, very few assess the ocular distribution of drugs or drug delivery systems (DDS). The latter is critical for the optimization of intraocular DDS design and drug bioavailability to achieve the desired ocular localization and duration of action (e.g., acute versus. prolonged). This study establishes the use of fiberoptic confocal laser microendoscopy (CLM) to qualitatively study the ocular distribution of fluorescent liposomes in real-time in live mice after sub-conjunctival injection. Being designed for in vivo visual inspection of tissues at the microscopic level, this is also the first full description of the CLM imaging method to study spatio-temporal distribution of injectables in the eye after subconjunctival injection.
URI: https://hdl.handle.net/10356/160751
ISSN: 1940-087X
DOI: 10.3791/62685
Schools: School of Materials Science and Engineering 
Organisations: National University of Singapore 
Rights: © 2021 JoVE Journal of Visualized Experiments. All rights reserved. This paper was published in JoVE Journal of Visualized Experiments and is made available with permission of JoVE Journal of Visualized Experiments.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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