Please use this identifier to cite or link to this item:
|Title:||Real-time nanoplasmonic sensing of three-dimensional morphological changes in a supported lipid bilayer and antimicrobial testing applications||Authors:||Yoon, Bo Kyeong
Zhdanov, Vladimir P.
Jackman, Joshua A.
|Keywords:||Engineering::Materials||Issue Date:||2021||Source:||Yoon, B. K., Park, H., Zhdanov, V. P., Jackman, J. A. & Cho, N. (2021). Real-time nanoplasmonic sensing of three-dimensional morphological changes in a supported lipid bilayer and antimicrobial testing applications. Biosensors & Bioelectronics, 174, 112768-. https://dx.doi.org/10.1016/j.bios.2020.112768||Journal:||Biosensors & Bioelectronics||Abstract:||We report the development of a real-time localized surface plasmon resonance (LSPR) biosensing strategy to detect three-dimensional morphological changes in a supported lipid bilayer (SLB) on a plasmonic substrate. The sensing concept advances on past efforts to detect subtle conformational changes in adsorbed biomacromolecules by demonstrating the capability to track large-scale, complex adsorbate shape changes and to classify different types of shape changes based on specific, multi-step measurement signatures. To validate this concept, we tested the addition of antimicrobial fatty acids, monoglycerides, and surfactants in micellar form to the SLB platform, which triggered specific three-dimensional membrane morphological changes such as tubule or bud formation along with solubilization. Experimentally, the different remodeling events were detected by distinct measurement signatures related to the shape and size of lipid protrusions that formed and evolved over time, which agreed well with a newly developed theoretical model. Our conceptual approach and formalism are applicable to various biosensing techniques, including not only LSPR but also surface plasmon resonance (SPR) and total internal reflection fluorescence (TIRF) microscopy. These sensing capabilities are advantageous for evaluating the mechanisms of antimicrobial drug candidates and other membrane-active compounds, and the measurement strategy is extendable to a wide range of biomimetic lipid compositions.||URI:||https://hdl.handle.net/10356/161144||ISSN:||2155-6210||DOI:||10.1016/j.bios.2020.112768||Schools:||School of Materials Science and Engineering||Rights:||© 2020 Elsevier B.V. All rights reserved.||Fulltext Permission:||none||Fulltext Availability:||No Fulltext|
|Appears in Collections:||MSE Journal Articles|
Updated on Dec 5, 2023
Web of ScienceTM
Updated on Oct 27, 2023
Updated on Dec 7, 2023
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.