Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/161243
Title: Identification of GABAergic neurons innervating the zebrafish lateral habenula
Authors: Ramaswamy, Mahathi
Cheng, Ruey-Kuang
Jesuthasan, Suresh
Keywords: Science::Medicine
Issue Date: 2020
Source: Ramaswamy, M., Cheng, R. & Jesuthasan, S. (2020). Identification of GABAergic neurons innervating the zebrafish lateral habenula. European Journal of Neuroscience, 52(8), 3918-3928. https://dx.doi.org/10.1111/ejn.14843
Project: MOE2017-T2-058
MOE2016-T1-001-152
Journal: European Journal of Neuroscience
Abstract: Habenula neurons are constantly active. The level of activity affects mood and behaviour, with increased activity in the lateral habenula reflecting exposure to punishment and a switch to passive coping and depression. Here, we identify GABAergic neurons that could reduce activity in the lateral habenula of larval zebrafish. GAD65/67 immunohistochemistry and imaging of gad1b:DsRed transgenic fish suggest the presence of GABAergic terminals in the neuropil and between cell bodies in the lateral habenula. Retrograde tracing with the lipophilic dye DiD suggests that the former derives from the thalamus, while the latter originates from a group of cells in the posterior hypothalamus that are located between the posterior tuberal nucleus and hypothalamic lobes. Two-photon calcium imaging indicates that blue light causes excitation of thalamic GABAergic neurons and terminals in the neuropil, while a subpopulation of lateral habenula neurons show reduced intracellular calcium levels. Whole-cell electrophysiological recording indicates that blue light reduces membrane potential of lateral habenula neurons. These observations suggest that GABAergic input from the thalamus may mediate inhibition in the zebrafish lateral habenula. Mechanisms governing release of GABA from the neurons in the posterior hypothalamus, which are likely to be in the tuberomammillary nucleus, remain to be defined.
URI: https://hdl.handle.net/10356/161243
ISSN: 0953-816X
DOI: 10.1111/ejn.14843
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: NUS Graduate School for Integrative Sciences and Engineering
Institute of Molecular and Cell Biology
Rights: © 2020 The Authors. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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