Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHa, Norberten_US
dc.contributor.authorDing, Nanen_US
dc.contributor.authorHong, Ruen_US
dc.contributor.authorLiu, Rubingen_US
dc.contributor.authorRoca, Xavieren_US
dc.contributor.authorLuo, Yingyuanen_US
dc.contributor.authorDuan, Xiaoweien_US
dc.contributor.authorWang, Xiaoen_US
dc.contributor.authorNi, Peilingen_US
dc.contributor.authorWu, Haiyangen_US
dc.contributor.authorZhang, Li-Fengen_US
dc.contributor.authorChen, Lingyien_US
dc.identifier.citationHa, N., Ding, N., Hong, R., Liu, R., Roca, X., Luo, Y., Duan, X., Wang, X., Ni, P., Wu, H., Zhang, L. & Chen, L. (2021). The lupus autoantigen La/Ssb is an Xist-binding protein involved in Xist folding and cloud formation. Nucleic Acids Research, 49(20), 11596-11613.
dc.description.abstractUsing the programmable RNA-sequence binding domain of the Pumilio protein, we FLAG-tagged Xist (inactivated X chromosome specific transcript) in live mouse cells. Affinity pulldown coupled to mass spectrometry was employed to identify a list of 138 candidate Xist-binding proteins, from which, Ssb (also known as the lupus autoantigen La) was validated as a protein functionally critical for X chromosome inactivation (XCI). Extensive XCI defects were detected in Ssb knockdown cells, including chromatin compaction, death of female mouse embryonic stem cells during in vitro differentiation and chromosome-wide monoallelic gene expression pattern. Live-cell imaging of Xist RNA reveals the defining XCI defect: Xist cloud formation. Ssb is a ubiquitous and versatile RNA-binding protein with RNA chaperone and RNA helicase activities. Functional dissection of Ssb shows that the RNA chaperone domain plays critical roles in XCI. In Ssb knockdown cells, Xist transcripts are unstable and misfolded. These results show that Ssb is critically involved in XCI, possibly as a protein regulating the in-cell structure of Xist.en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipMinistry of Health (MOH)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.relation.ispartofNucleic Acids Researchen_US
dc.rights© The Authors 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleThe lupus autoantigen La/Ssb is an Xist-binding protein involved in Xist folding and cloud formationen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsBinding Proteinen_US
dc.subject.keywordsAnimal Cellen_US
dc.description.acknowledgementL.C. was supported by the National Key R&D Program of China [2018YFC1313003, 2018YFA0107002]; National Natural Science Foundation of China [31871485]; Natural Science Foundation of Tianjin [18JCJQJC48400]; 111 Project Grant [B08011]; Fundamental Research Funds for the Central Universities; L.-F.Z. was supported by Singapore Ministry of Education Academic Research Fund [MOE2015-T2-1-093]; Singapore National Research Foundation under its Cooperative Basic Research Grant administered by the Singapore Ministry of Health’s National Medical Research Council [NMRC/CBRG/0092/2015]. Funding for open access charge: the National Key R&D Program of China [2018YFA0107002].en_US
item.fulltextWith Fulltext-
Appears in Collections:SBS Journal Articles
Files in This Item:
File Description SizeFormat 
gkab1003.pdf6.66 MBAdobe PDFThumbnail

Citations 50

Updated on Nov 25, 2023

Page view(s)

Updated on Nov 28, 2023

Download(s) 50

Updated on Nov 28, 2023

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.