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dc.contributor.authorOluwabusola, Emmanuel T.en_US
dc.contributor.authorKatermeran, Nursheena Parveenen_US
dc.contributor.authorPoh, Wee Hanen_US
dc.contributor.authorGoh, Ben Teo Minen_US
dc.contributor.authorTan, Lik Tongen_US
dc.contributor.authorDiyaolu, Oluwatofunmilayoen_US
dc.contributor.authorTabudravu, Jiojien_US
dc.contributor.authorEbel, Raineren_US
dc.contributor.authorRice, Scott A.en_US
dc.contributor.authorJaspars, Marcelen_US
dc.identifier.citationOluwabusola, E. T., Katermeran, N. P., Poh, W. H., Goh, B. T. M., Tan, L. T., Diyaolu, O., Tabudravu, J., Ebel, R., Rice, S. A. & Jaspars, M. (2022). Inhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasin. Molecules, 27(5), 1721-.
dc.description.abstractNatural products derived from marine sponges have exhibited bioactivity and, in some cases, serve as potent quorum sensing inhibitory agents that prevent biofilm formation and attenuate virulence factor expression by pathogenic microorganisms. In this study, the inhibitory activity of the psammaplin-type compounds, psammaplin A (1) and bisaprasin (2), isolated from the marine sponge, Aplysinellarhax, are evaluated in quorum sensing inhibitory assays based on the Pseudomonas aeruginosa PAO1 lasB-gfp(ASV) and rhlA-gfp(ASV) biosensor strains. The results indicate that psammaplin A (1) showed moderate inhibition on lasB-gfp expression, but significantly inhibited the QS-gene promoter, rhlA-gfp, with IC50 values at 14.02 μM and 4.99 μM, respectively. In contrast, bisaprasin (2) displayed significant florescence inhibition in both biosensors, PAO1 lasB-gfp and rhlA-gfp, with IC50 values at 3.53 μM and 2.41 μM, respectively. Preliminary analysis suggested the importance of the bromotyrosine and oxime functionalities for QSI activity in these molecules. In addition, psammaplin A and bisaprasin downregulated elastase expression as determined by the standard enzymatic elastase assay, although greater reduction in elastase production was observed with 1 at 50 μM and 100 μM. Furthermore, the study revealed that bisaprasin (2) reduced biofilm formation in P. aeruginosa.en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.rights© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).en_US
dc.subjectScience::Biological sciencesen_US
dc.titleInhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasinen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.researchSingapore Centre for Environmental Life Sciences and Engineering (SCELSE)en_US
dc.description.versionPublished versionen_US
dc.subject.keywordsMarine Spongeen_US
dc.description.acknowledgementThe facilities and equipment support were provided partially by the National Institute of Education, Singapore. This research is funded by the National Research Foundation, Prime Minister’s Office, Singapore under its Marine Science Research and Development Program (Award Nos. MSRDP-P15 and MSRDP-P34). In addition, we acknowledge the E.U. Seventh Framework Programme Project PharmaSea (grant agreement no-312184) for financial support for the collection of the marine sample.en_US
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