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Title: Inhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasin
Authors: Oluwabusola, Emmanuel T.
Katermeran, Nursheena Parveen
Poh, Wee Han
Goh, Ben Teo Min
Tan, Lik Tong
Diyaolu, Oluwatofunmilayo
Tabudravu, Jioji
Ebel, Rainer
Rice, Scott A.
Jaspars, Marcel
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Oluwabusola, E. T., Katermeran, N. P., Poh, W. H., Goh, B. T. M., Tan, L. T., Diyaolu, O., Tabudravu, J., Ebel, R., Rice, S. A. & Jaspars, M. (2022). Inhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasin. Molecules, 27(5), 1721-.
Project: MSRDP-P15 
Journal: Molecules 
Abstract: Natural products derived from marine sponges have exhibited bioactivity and, in some cases, serve as potent quorum sensing inhibitory agents that prevent biofilm formation and attenuate virulence factor expression by pathogenic microorganisms. In this study, the inhibitory activity of the psammaplin-type compounds, psammaplin A (1) and bisaprasin (2), isolated from the marine sponge, Aplysinellarhax, are evaluated in quorum sensing inhibitory assays based on the Pseudomonas aeruginosa PAO1 lasB-gfp(ASV) and rhlA-gfp(ASV) biosensor strains. The results indicate that psammaplin A (1) showed moderate inhibition on lasB-gfp expression, but significantly inhibited the QS-gene promoter, rhlA-gfp, with IC50 values at 14.02 μM and 4.99 μM, respectively. In contrast, bisaprasin (2) displayed significant florescence inhibition in both biosensors, PAO1 lasB-gfp and rhlA-gfp, with IC50 values at 3.53 μM and 2.41 μM, respectively. Preliminary analysis suggested the importance of the bromotyrosine and oxime functionalities for QSI activity in these molecules. In addition, psammaplin A and bisaprasin downregulated elastase expression as determined by the standard enzymatic elastase assay, although greater reduction in elastase production was observed with 1 at 50 μM and 100 μM. Furthermore, the study revealed that bisaprasin (2) reduced biofilm formation in P. aeruginosa.
ISSN: 1420-3049
DOI: 10.3390/molecules27051721
Schools: School of Biological Sciences 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SCELSE Journal Articles

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