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|Title:||Inhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasin||Authors:||Oluwabusola, Emmanuel T.
Katermeran, Nursheena Parveen
Poh, Wee Han
Goh, Ben Teo Min
Tan, Lik Tong
Rice, Scott A.
|Keywords:||Science::Biological sciences||Issue Date:||2022||Source:||Oluwabusola, E. T., Katermeran, N. P., Poh, W. H., Goh, B. T. M., Tan, L. T., Diyaolu, O., Tabudravu, J., Ebel, R., Rice, S. A. & Jaspars, M. (2022). Inhibition of the quorum sensing system, elastase production and biofilm formation in pseudomonas aeruginosa by psammaplin A and bisaprasin. Molecules, 27(5), 1721-. https://dx.doi.org/10.3390/molecules27051721||Project:||MSRDP-P15
|Journal:||Molecules||Abstract:||Natural products derived from marine sponges have exhibited bioactivity and, in some cases, serve as potent quorum sensing inhibitory agents that prevent biofilm formation and attenuate virulence factor expression by pathogenic microorganisms. In this study, the inhibitory activity of the psammaplin-type compounds, psammaplin A (1) and bisaprasin (2), isolated from the marine sponge, Aplysinellarhax, are evaluated in quorum sensing inhibitory assays based on the Pseudomonas aeruginosa PAO1 lasB-gfp(ASV) and rhlA-gfp(ASV) biosensor strains. The results indicate that psammaplin A (1) showed moderate inhibition on lasB-gfp expression, but significantly inhibited the QS-gene promoter, rhlA-gfp, with IC50 values at 14.02 μM and 4.99 μM, respectively. In contrast, bisaprasin (2) displayed significant florescence inhibition in both biosensors, PAO1 lasB-gfp and rhlA-gfp, with IC50 values at 3.53 μM and 2.41 μM, respectively. Preliminary analysis suggested the importance of the bromotyrosine and oxime functionalities for QSI activity in these molecules. In addition, psammaplin A and bisaprasin downregulated elastase expression as determined by the standard enzymatic elastase assay, although greater reduction in elastase production was observed with 1 at 50 μM and 100 μM. Furthermore, the study revealed that bisaprasin (2) reduced biofilm formation in P. aeruginosa.||URI:||https://hdl.handle.net/10356/161302||ISSN:||1420-3049||DOI:||10.3390/molecules27051721||Schools:||School of Biological Sciences||Research Centres:||Singapore Centre for Environmental Life Sciences and Engineering (SCELSE)||Rights:||© 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/).||Fulltext Permission:||open||Fulltext Availability:||With Fulltext|
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