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Title: Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis
Authors: Qiao, Zhu
Kong, Que
Tee, Wan Ting
Lim, Audrey R. Q.
Teo, Miao Xuan
Olieric, Vincent
Low, Pui Man
Yang, Yuzhou
Qian, Guoliang
Ma, Wei
Gao, Yong-Gui
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Qiao, Z., Kong, Q., Tee, W. T., Lim, A. R. Q., Teo, M. X., Olieric, V., Low, P. M., Yang, Y., Qian, G., Ma, W. & Gao, Y. (2022). Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis. Science Advances, 8(34), eabq1211-.
Project: MOE-2019-T2-099
Journal: Science Advances
Abstract: Vegetable oils are not only major components of human diet but also vital for industrial applications. WRINKLED1 (WRI1) is a pivotal transcription factor governing plant oil biosynthesis, but the underlying DNA-binding mechanism remains incompletely understood. Here, we resolved the structure of Arabidopsis WRI1 (AtWRI1) with its cognate double-stranded DNA (dsDNA), revealing two antiparallel β sheets in the tandem AP2 domains that intercalate into the adjacent major grooves of dsDNA to determine the sequence recognition specificity. We showed that AtWRI1 represented a previously unidentified structural fold and DNA-binding mode. Mutations of the key residues interacting with DNA element affected its binding affinity and oil biosynthesis when these variants were transiently expressed in tobacco leaves. Seed oil content was enhanced in stable transgenic wri1-1 expressing an AtWRI1 variant (W74R). Together, our findings offer a structural basis explaining WRI1 recognition and binding of DNA and suggest an alternative strategy to increase oil yield in crops through WRI1 bioengineering.
ISSN: 2375-2548
DOI: 10.1126/sciadv.abq1211
Schools: School of Biological Sciences 
Research Centres: NTU Institute of Structural Biology 
Rights: © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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