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Title: Characterisation of Salmonella Enteritidis ST11 and ST1925 associated with human intestinal and extra-intestinal infections in Singapore
Authors: Aung, Kyaw Thu
Khor, Wei Ching
Ong, Kar Hui
Tan, Wei Ling
Wong, Zhi Ning
Oh, Jia Quan
Wong, Wai Kwan
Tan, Brian Zi Yan
Maiwald, Matthias
Tee, Nancy Wen Sim
Barkham, Timothy
Koh, Tse Hsien
Dalsgaard, Anders
Chen, Swaine L.
Schlundt, Joergen
Ng, Lee Ching
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Aung, K. T., Khor, W. C., Ong, K. H., Tan, W. L., Wong, Z. N., Oh, J. Q., Wong, W. K., Tan, B. Z. Y., Maiwald, M., Tee, N. W. S., Barkham, T., Koh, T. H., Dalsgaard, A., Chen, S. L., Schlundt, J. & Ng, L. C. (2022). Characterisation of Salmonella Enteritidis ST11 and ST1925 associated with human intestinal and extra-intestinal infections in Singapore. International Journal of Environmental Research and Public Health, 19(9), 5671-.
Journal: International Journal of Environmental Research and Public Health 
Abstract: Salmonella Enteritidis is a major foodborne pathogen worldwide. In this study, a total of 276 S. enteritidis isolates, collected between 2016 and 2017 from human, food and farm/slaughterhouse samples, were studied to enhance the understanding of the epidemiology of human salmonellosis in Singapore. Results showed all 276 isolates belonged either to ST1925 (70.3%) or ST11 (29.7%), with ST11 being significantly more frequent in extra-intestinal isolates and chicken isolates. Food isolates, most of which were from poultry, showed the highest prevalence of resistance (33-37%) against beta-lactams or beta-lactams/beta-lactamase inhibitor combination (ampicillin, piperacillin and ampicillin/sulbactam). The analysis showed the detection of genes associated with resistance to aminoglycoside genes (99.6%), tetracycline (55.1%), and beta-lactams (14.9%) of all isolates. Nine types of plasmids were found in 266 isolates; the most common incompatibility group profiles were IncFIB(S)-IncFII(S)-IncX1 (72.2%) and IncFIB(S)-IncFII(S) (15.8%). Most plasmid harbouring isolates from chicken (63.6%, 14/22) and from human (73.8%, 175/237) shared the same plasmid profile (IncFIB(S)-IncFII(S)-IncX1). SNP analysis showed clustering of several isolates from poultry food products and human isolates, suggesting phylogenetic relatedness among these isolates. Lastly, this study provides important epidemiological insights on the application of phenotypic and next-generation sequencing (NGS) tools for improved food safety and public health surveillance and outbreak investigation of S.enteritidis.
ISSN: 1661-7827
DOI: 10.3390/ijerph19095671
Schools: School of Chemical and Biomedical Engineering 
School of Biological Sciences 
Organisations: National Centre for Food Science, Singapore Food Agency
Environmental Health Institute, National Environment Agency
Research Centres: Nanyang Technological University Food Technology Centre
Rights: © 2022 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles
SCBE Journal Articles

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