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Title: Insights into the mechanism, structure and potential as drug target of the mycobacterial bd-type terminal oxidase
Authors: Ekaterina, Sviriaeva
Keywords: Science::Biological sciences::Microbiology
Issue Date: 2022
Publisher: Nanyang Technological University
Source: Ekaterina, S. (2022). Insights into the mechanism, structure and potential as drug target of the mycobacterial bd-type terminal oxidase. Doctoral thesis, Nanyang Technological University, Singapore.
Project: NRF-CRP18-2017- 01 
Abstract: Mycobacterium tuberculosis, the causative agent of tuberculosis, possesses two terminal oxidases, the cytochrome bcc:aa3 (cyt-bcc:aa3) and the cytochrome bd (cyt-bd). While the cyt-bcc:aa3 is the target of the drug candidate Q203, the cyt-bd remains poorly understood. This work aimed to study the structure and function of the mycobacterial cyt-bd. Site-directed mutagenesis was used to identify unique functionally important regions of the cyt-bd. Attempts to purify the cyt-bd to resolve its structure were made. However, the complex could not be obtained in its intact form despite multiple attempts and optimizations. The involvement of the cyt-bd in the synergy between Q203 and clofazimine, an antitubercular drug, was evaluated, leading to a better understanding of the complex modes of action of clofazimine. In conclusion, the work presented in this thesis contributes to knowledge about the mycobacterial cyt-bd, from its structural features to its role in modulating potency of drugs, such as clofazimine.
DOI: 10.32657/10356/161580
Schools: School of Biological Sciences 
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: embargo_20240908
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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