Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/161603
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dc.contributor.authorTay, Hui Minen_US
dc.contributor.authorYeap, Wei Hseunen_US
dc.contributor.authorDalan, Rinkooen_US
dc.contributor.authorWong, Siew Chengen_US
dc.contributor.authorHou, Han Weien_US
dc.date.accessioned2022-09-09T07:40:53Z-
dc.date.available2022-09-09T07:40:53Z-
dc.date.issued2020-
dc.identifier.citationTay, H. M., Yeap, W. H., Dalan, R., Wong, S. C. & Hou, H. W. (2020). Increased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiency. FASEB Journal, 34(8), 11133-11142. https://dx.doi.org/10.1096/fj.202000822Ren_US
dc.identifier.issn0892-6638en_US
dc.identifier.urihttps://hdl.handle.net/10356/161603-
dc.description.abstractVitamin D deficiency is a major public health problem worldwide, linked to several chronic diseases including cardiovascular diseases. While immunomodulatory effects of vitamin D on monocytes have been reported in cardiovascular and metabolic diseases, there is limited understanding on monocyte phenotype in healthy individuals with suboptimal vitamin D levels and without any clinical diseases. In this work, we performed label-free, microfluidic isolation of monocytes, and characterized their functional phenotype using flow cytometry and in vitro vascular models in healthy subjects with (n = 7) and without vitamin D deficiency (n = 16). Vitamin D deficient (VitD-Def) subjects (25(OH)D3 level < 26 ng/mL) expressed significant downregulation of vitamin D receptor (VDR) on monocytes as compared to controls (P < .0001), and VDR expression was well-associated with serum 25(OH)D3 levels. Increased monocyte-platelet aggregates (MPA), a marker for platelet activation, were also observed in VitD-Def subjects (P < .05) which suggests a pro-inflammatory monocyte phenotype. Monocyte adhesion to endothelial cells, an early-stage atherosclerosis event, was also higher in VitD-Def individuals, and inversely correlated to serum 25(OH)D3 level (P < .05). Taken together, these results indicate the pro-inflammatory state and atherogenic potential of monocytes in VitD-Def healthy subjects, and propound the use of vitamin D supplementation as a prospective immunomodulatory and anti-inflammatory therapy in atherosclerosis.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipMinistry of Health (MOH)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.language.isoenen_US
dc.relationNMRC/BNIG/2038/2015en_US
dc.relationRG53/18en_US
dc.relation.ispartofFASEB Journalen_US
dc.rights© 2020 Federation of American Societies for Experimental Biology. All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleIncreased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiencyen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationSingapore Immunology Network, A*STARen_US
dc.contributor.organizationTan Tock Seng Hospitalen_US
dc.identifier.doi10.1096/fj.202000822R-
dc.identifier.pmid32627899-
dc.identifier.scopus2-s2.0-85087483735-
dc.identifier.issue8en_US
dc.identifier.volume34en_US
dc.identifier.spage11133en_US
dc.identifier.epage11142en_US
dc.subject.keywordsAtherosclerosisen_US
dc.subject.keywordsMicrofluidicsen_US
dc.description.acknowledgementThis research is supported by the National Research Foundation Singapore under its CBRG-NIG (NMRC/BNIG/2038/2015) and administered by the Singapore Ministry of Health's, National Medical Research Council and Biomedical Research Council (BMRC), ASTAR. HWH would like to acknowledge support from an NTU start-up grant and the Singapore Ministry of Education (MOE) Academic Research Fund Tier 1 (RG53/18).en_US
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