Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorTay, Hui Minen_US
dc.contributor.authorYeap, Wei Hseunen_US
dc.contributor.authorDalan, Rinkooen_US
dc.contributor.authorWong, Siew Chengen_US
dc.contributor.authorHou, Han Weien_US
dc.identifier.citationTay, H. M., Yeap, W. H., Dalan, R., Wong, S. C. & Hou, H. W. (2020). Increased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiency. FASEB Journal, 34(8), 11133-11142.
dc.description.abstractVitamin D deficiency is a major public health problem worldwide, linked to several chronic diseases including cardiovascular diseases. While immunomodulatory effects of vitamin D on monocytes have been reported in cardiovascular and metabolic diseases, there is limited understanding on monocyte phenotype in healthy individuals with suboptimal vitamin D levels and without any clinical diseases. In this work, we performed label-free, microfluidic isolation of monocytes, and characterized their functional phenotype using flow cytometry and in vitro vascular models in healthy subjects with (n = 7) and without vitamin D deficiency (n = 16). Vitamin D deficient (VitD-Def) subjects (25(OH)D3 level < 26 ng/mL) expressed significant downregulation of vitamin D receptor (VDR) on monocytes as compared to controls (P < .0001), and VDR expression was well-associated with serum 25(OH)D3 levels. Increased monocyte-platelet aggregates (MPA), a marker for platelet activation, were also observed in VitD-Def subjects (P < .05) which suggests a pro-inflammatory monocyte phenotype. Monocyte adhesion to endothelial cells, an early-stage atherosclerosis event, was also higher in VitD-Def individuals, and inversely correlated to serum 25(OH)D3 level (P < .05). Taken together, these results indicate the pro-inflammatory state and atherogenic potential of monocytes in VitD-Def healthy subjects, and propound the use of vitamin D supplementation as a prospective immunomodulatory and anti-inflammatory therapy in atherosclerosis.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.description.sponsorshipMinistry of Health (MOH)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.relation.ispartofFASEB Journalen_US
dc.rights© 2020 Federation of American Societies for Experimental Biology. All rights reserved.en_US
dc.subjectScience::Biological sciencesen_US
dc.titleIncreased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiencyen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Mechanical and Aerospace Engineeringen_US
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationSingapore Immunology Network, A*STARen_US
dc.contributor.organizationTan Tock Seng Hospitalen_US
dc.description.acknowledgementThis research is supported by the National Research Foundation Singapore under its CBRG-NIG (NMRC/BNIG/2038/2015) and administered by the Singapore Ministry of Health's, National Medical Research Council and Biomedical Research Council (BMRC), ASTAR. HWH would like to acknowledge support from an NTU start-up grant and the Singapore Ministry of Education (MOE) Academic Research Fund Tier 1 (RG53/18).en_US
item.fulltextNo Fulltext-
Appears in Collections:LKCMedicine Journal Articles
MAE Journal Articles
SBS Journal Articles

Citations 20

Updated on Feb 20, 2024

Web of ScienceTM
Citations 20

Updated on Oct 25, 2023

Page view(s)

Updated on Feb 16, 2024

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.