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Title: Increased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiency
Authors: Tay, Hui Min
Yeap, Wei Hseun
Dalan, Rinkoo
Wong, Siew Cheng
Hou, Han Wei
Keywords: Science::Biological sciences
Issue Date: 2020
Source: Tay, H. M., Yeap, W. H., Dalan, R., Wong, S. C. & Hou, H. W. (2020). Increased monocyte-platelet aggregates and monocyte-endothelial adhesion in healthy individuals with vitamin D deficiency. FASEB Journal, 34(8), 11133-11142.
Project: NMRC/BNIG/2038/2015
Journal: FASEB Journal
Abstract: Vitamin D deficiency is a major public health problem worldwide, linked to several chronic diseases including cardiovascular diseases. While immunomodulatory effects of vitamin D on monocytes have been reported in cardiovascular and metabolic diseases, there is limited understanding on monocyte phenotype in healthy individuals with suboptimal vitamin D levels and without any clinical diseases. In this work, we performed label-free, microfluidic isolation of monocytes, and characterized their functional phenotype using flow cytometry and in vitro vascular models in healthy subjects with (n = 7) and without vitamin D deficiency (n = 16). Vitamin D deficient (VitD-Def) subjects (25(OH)D3 level < 26 ng/mL) expressed significant downregulation of vitamin D receptor (VDR) on monocytes as compared to controls (P < .0001), and VDR expression was well-associated with serum 25(OH)D3 levels. Increased monocyte-platelet aggregates (MPA), a marker for platelet activation, were also observed in VitD-Def subjects (P < .05) which suggests a pro-inflammatory monocyte phenotype. Monocyte adhesion to endothelial cells, an early-stage atherosclerosis event, was also higher in VitD-Def individuals, and inversely correlated to serum 25(OH)D3 level (P < .05). Taken together, these results indicate the pro-inflammatory state and atherogenic potential of monocytes in VitD-Def healthy subjects, and propound the use of vitamin D supplementation as a prospective immunomodulatory and anti-inflammatory therapy in atherosclerosis.
ISSN: 0892-6638
DOI: 10.1096/fj.202000822R
Schools: School of Mechanical and Aerospace Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
School of Biological Sciences 
Organisations: Singapore Immunology Network, A*STAR
Tan Tock Seng Hospital
Rights: © 2020 Federation of American Societies for Experimental Biology. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles
MAE Journal Articles
SBS Journal Articles

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