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Title: Liquorilactobacillus satsumensis from water kefir yields α-glucan polysaccharides with prebiotic and synbiotic qualities
Authors: Tan, Li Ling
Ngiam, Jia Jun
Sim, Edric Shao Zhe
Conway, Patricia Lynne
Loo, Joachim Say Chye
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Tan, L. L., Ngiam, J. J., Sim, E. S. Z., Conway, P. L. & Loo, J. S. C. (2022). Liquorilactobacillus satsumensis from water kefir yields α-glucan polysaccharides with prebiotic and synbiotic qualities. Carbohydrate Polymers, 290, 119515-.
Project: MOE/RCE: M4330019.C70 
Journal: Carbohydrate Polymers 
Abstract: Exopolysaccharides from water kefir grains are a potential source of novel, food-safe and functional materials. Herein, prebiotic properties of polysaccharides produced by water kefir-derived Liquorilactobacillus satsumensis bacteria were explored. Strains were cultured in sucrose-supplemented media for exopolysaccharides production, and partial hydrolysis was performed to yield shorter chain polysaccharides. Structural characterization revealed that hydrolyzed polysaccharides were branched glucans comprising α-1,6 bonds and α-1,3/α-1,4 branching, with molecular weight of ~10 kDa. Hydrolyzed polysaccharides demonstrated selective utilization by probiotics, but not by pathogens, and were non-digestible by human digestive enzymes. Particularly, hydrolyzed polysaccharides were fermentable by kefir-derived probiotics, and these were combined in a novel kefir synbiotic formulation. Using large bowel simulated conditions, it was demonstrated that hydrolyzed polysaccharides and kefir synbiotics promoted Bacteroidetes abundance, and increased acetate, propionate, and butyrate concentrations. Overall, hydrolyzed glucans from Liquorilactobacillus satsumensis have prebiotic properties with enhanced benefits in a synbiotic when combined with kefir probiotics.
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2022.119515
Schools: School of Materials Science and Engineering 
School of Biological Sciences 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering (SCELSE) 
Rights: © 2022 Elsevier Ltd. All rights reserved. This paper was published in Carbohydrate Polymers and is made available with permission of Elsevier Ltd.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MSE Journal Articles
SBS Journal Articles
SCELSE Journal Articles

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