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Title: In situ alginate crosslinking during spray-drying of lactobacilli probiotics promotes gastrointestinal-targeted delivery
Authors: Tan, Li Ling
Mahotra, Manish
Chan, Si Ye
Loo, Joachim Say Chye
Keywords: Engineering::Materials
Issue Date: 2022
Source: Tan, L. L., Mahotra, M., Chan, S. Y. & Loo, J. S. C. (2022). In situ alginate crosslinking during spray-drying of lactobacilli probiotics promotes gastrointestinal-targeted delivery. Carbohydrate Polymers, 286, 119279-.
Project: MOE/RCE: M4330019.C70 
Journal: Carbohydrate Polymers 
Abstract: Alginate-based formulations have shown desirable functional characteristics for probiotic encapsulation. However, current technologies used to produce these formulations are inefficient, detrimental to probiotics viability or do not produce dry, shelf-stable products. Herein, we developed a novel spray-drying technique that combines particle formation, alginate crosslinking and drying into a single step, thereby streamlining the production of encapsulated probiotics powder. Lacticaseibacillus rhamnosus GG (LGG) encapsulated in six encapsulation formulations were characterized and compared. Among the six formulations investigated, the crosslinked alginate with sucrose formulation (Ca-Alg-Suc) was found to be most promising, achieving ~109 CFU/g of surviving LGG after spray-drying and exposure to simulated gastric fluid (SGF). The Ca-Alg-Suc formulation was further evaluated with Lactiplantibacillus plantarum and Lacticaseibacillus paracasei, and similar results of high post-spray-drying and post-SGF viabilities were obtained. Successful encapsulation of different lactobacilli probiotics via the proposed spray-drying technique highlights potential of this procedure to be scaled up for commercial applications.
ISSN: 0144-8617
DOI: 10.1016/j.carbpol.2022.119279
DOI (Related Dataset): 10.21979/N9/B0Q7BG
Schools: School of Materials Science and Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering 
Rights: © 2022 Elsevier Ltd. All rights reserved. This paper was published in Carbohydrate Polymers and is made available with permission of Elsevier Ltd.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
MSE Journal Articles
SCELSE Journal Articles

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