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Title: Colorimetric and fluorometric profiling of advanced glycation end products
Authors: Ammanath, Gopal
Delachi, Carla Giorgia
Karabacak, Soner
Ali, Yusuf
Boehm, Bernhard Otto
Yildiz, Umit Hakan
Alagappan, Palaniappan
Liedberg, Bo
Keywords: Engineering::Materials
Issue Date: 2022
Source: Ammanath, G., Delachi, C. G., Karabacak, S., Ali, Y., Boehm, B. O., Yildiz, U. H., Alagappan, P. & Liedberg, B. (2022). Colorimetric and fluorometric profiling of advanced glycation end products. ACS Applied Materials & Interfaces, 14(1), 94-103.
Project: RG 82/12
Journal: ACS Applied Materials & Interfaces 
Abstract: Profiling of advanced glycation end products (AGEs) is an emerging area of clinical significance for disease diagnosis and prognosis. Typically, concentrations of AGEs are estimated in laboratories by trained personnel using sophisticated equipment. Herein, a facile approach for colorimetric and fluorometric profiling of AGEs is reported for rapid and on-site analysis. The concentrations of AGE levels in plasma are estimated via changes in optical properties of polythiophenes (PTs) upon interaction with aptamers (Apts) in the presence and in the absence of AGEs. To validate the proposed approach, glyceraldehyde-derived AGEs (AGE class 1 [AGE1]), the biomarker associated with cardiovascular diseases and diabetes, are used as a model system. Colorimetric analysis yielded linear responses for AGE1 for clinically relevant concentration ranges between 1.5 and 300 μg/mL with a limit of detection (LOD) of ∼1.3 μg/mL. Subsequently, an approach utilizing PTs with four different pendant groups in conjunction with four different Apts is demonstrated for qualitative colorimetric profiling and for quantitative fluorometric profiling of up to four AGEs in clinical matrices. Principal component analysis (PCA) of fluorometric responses of AGE-spiked samples yielded distinct responses for the different AGEs tested. Thus, the proposed approach ascertains rapid profiling of spiked AGEs in plasma samples without the requirement of preanalytical processing and advanced instrumentation, thereby facilitating on-site diagnosis.
ISSN: 1944-8244
DOI: 10.1021/acsami.1c16261
Schools: School of Materials Science and Engineering 
Lee Kong Chian School of Medicine (LKCMedicine) 
Research Centres: Centre for Biomimetic Sensor Science (CBSS) 
Rights: © 2021 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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