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Title: Self-assembly of a barnacle cement protein (MrCP20) into adhesive nanofibrils with concomitant regulation of CaCO₃ polymorphism
Authors: Mohanram, Harini
Georges, Tristan
Pervushin, Konstantin
Azaïs, Thierry
Miserez, Ali
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Mohanram, H., Georges, T., Pervushin, K., Azaïs, T. & Miserez, A. (2021). Self-assembly of a barnacle cement protein (MrCP20) into adhesive nanofibrils with concomitant regulation of CaCO₃ polymorphism. Chemistry of Materials, 33(24), 9715-9724.
Project: MOE 2019-T3-1-012
Journal: Chemistry of Materials
Abstract: Barnacles are convenient model organisms to study both biomineralization and bioadhesion phenomena. They secrete a proteinaceous adhesive from cement proteins (CPs) to strongly attach to solid immersed substrates. More recently, it has been suggested that some CPs also play a key role in regulating the calcification of barnacles’ protective shells. In this study, combining both solution and solid-state NMR spectroscopy, Raman and infrared spectroscopy studies, and atomic force microscopy (AFM) and transmission electron microscopy (TEM) imaging, we have explored the CaCO3 mineralization pathway regulated by Megabalanus rosa CP 20 (MrCP20). Our data show that MrCP20 can sequestrate inorganic calcium and carbonate ions from the solution state, which quickly coarsen into liquid-like microdroplets and subsequently form protovaterite amorphous CaCO3 (ACC) particles. This pathway leads to the stabilization of the metastable vaterite polymorphism of CaCO3. Simultaneously, AFM and TEM investigations show that MrCP20 undergoes fibrillization triggered by a pH drift arising during CaCO3 mineralization, leading to amyloid-like nanofibrils. Based on protein NMR, this mechanism appears to be stabilized by the reduction of intramolecular disulfide bonds. Collectively, our results demonstrate that MrCP20 plays a synergistic role of regulating CaCO3 biomineralization while concomitantly self-assembling into adhesive nanofibrils.
ISSN: 0897-4756
DOI: 10.1021/acs.chemmater.1c03477
Schools: School of Biological Sciences 
School of Materials Science and Engineering 
Research Centres: Center for Sustainable Materials (SusMat)
Rights: © 2021 American Chemical Society. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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