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Title: Human PERK rescues unfolded protein response-deficient yeast cells
Authors: Yap, Wei Sheng
Thibault, Guillaume
Keywords: Science::Medicine
Issue Date: 2022
Source: Yap, W. S. & Thibault, G. (2022). Human PERK rescues unfolded protein response-deficient yeast cells. MicroPublication Biology, 2022.
Project: NRF2018NRFNSFC003SB-006
Journal: microPublication biology
Abstract: Protein folding and quality control is tightly regulated at the endoplasmic reticulum (ER), and its disruption is associated with many diseases. In eukaryotes, the accumulation of unfolded protein in the ER is sensed by the three sensors, IRE1, PERK, and ATF6 to activate the unfolded protein response (UPR) to restore ER homeostasis. However, uncoupling the sensing of each sensor and their respective downstream pathways has been challenging as the absence of one is compensated by the remaining two sensors. Here, we report a fully functional human PERK (hPERK) chimeric protein expressed in Saccharomyces cerevisiae that could be used for high throughput screen to identify new PERK inhibitory or activating compounds as well as to characterize the PERK stress sensing mechanisms.
ISSN: 2578-9430
DOI: 10.17912/micropub.biology.000592
DOI (Related Dataset): 10.21979/N9/IGLQOE
Schools: School of Biological Sciences 
Organisations: Institute of Molecular and Cell Biology, A*STAR
Rights: © 2022 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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