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Title: Functionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteria
Authors: Tan, Jie Ren
Xing, Bengang
Lee, Ken Chi-Lik
Keywords: Science::Chemistry
Issue Date: 2022
Source: Tan, J. R., Ferdinandus, Xing, B. & Lee, K. C. (2022). Functionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteria. Sensors and Actuators B: Chemical, 367, 132123-.
Project: RT15/19
Journal: Sensors and Actuators B: Chemical
Abstract: Biosensors for rapid and sensitive detection of pathogens play a crucial role in healthcare and food safety. Despite the development of numerous sensing devices, it remains a challenge to obtain biosensors that are reliable, easy-to-use and can offer results within minutes. Herein, we report a functionalized liposome, with an embedded fluorescent dye to offer thermoswitchable emission, for selective Gram-negative bacteria detection. This targeting system utilizes polymyxin B (PmB) as a recognition element, where it can be conjugated onto the surface of the liposome to differentiate Gram-negative bacteria from Gram-positive counterparts as well as mammalian cells via fluorescence spectroscopy. The unique thermoswitchable fluorescence conferred through manipulation of aggregation-induced quenching allows for high signal-to-noise detection, reaching up to 3.5-fold increase in emission in the case of E. coli. This is in contrast to typical small organic fluorescent labels and future work includes developing a suitable capture probe to achieve lower limits of detection.
ISSN: 0925-4005
DOI: 10.1016/j.snb.2022.132123
Rights: © 2022 Elsevier B.V. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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