Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/162040
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dc.contributor.authorTan, Jie Renen_US
dc.contributor.authorFerdinandusen_US
dc.contributor.authorXing, Bengangen_US
dc.contributor.authorLee, Ken Chi-Liken_US
dc.date.accessioned2022-10-03T01:04:26Z-
dc.date.available2022-10-03T01:04:26Z-
dc.date.issued2022-
dc.identifier.citationTan, J. R., Ferdinandus, Xing, B. & Lee, K. C. (2022). Functionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteria. Sensors and Actuators B: Chemical, 367, 132123-. https://dx.doi.org/10.1016/j.snb.2022.132123en_US
dc.identifier.issn0925-4005en_US
dc.identifier.urihttps://hdl.handle.net/10356/162040-
dc.description.abstractBiosensors for rapid and sensitive detection of pathogens play a crucial role in healthcare and food safety. Despite the development of numerous sensing devices, it remains a challenge to obtain biosensors that are reliable, easy-to-use and can offer results within minutes. Herein, we report a functionalized liposome, with an embedded fluorescent dye to offer thermoswitchable emission, for selective Gram-negative bacteria detection. This targeting system utilizes polymyxin B (PmB) as a recognition element, where it can be conjugated onto the surface of the liposome to differentiate Gram-negative bacteria from Gram-positive counterparts as well as mammalian cells via fluorescence spectroscopy. The unique thermoswitchable fluorescence conferred through manipulation of aggregation-induced quenching allows for high signal-to-noise detection, reaching up to 3.5-fold increase in emission in the case of E. coli. This is in contrast to typical small organic fluorescent labels and future work includes developing a suitable capture probe to achieve lower limits of detection.en_US
dc.description.sponsorshipAgency for Science, Technology and Research (A*STAR)en_US
dc.description.sponsorshipMinistry of Education (MOE)en_US
dc.language.isoenen_US
dc.relationRT15/19en_US
dc.relationRG6/20en_US
dc.relationA1983c0028en_US
dc.relationA20E5c0090en_US
dc.relation.ispartofSensors and Actuators B: Chemicalen_US
dc.rights© 2022 Elsevier B.V. All rights reserved.en_US
dc.subjectScience::Chemistryen_US
dc.titleFunctionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteriaen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.identifier.doi10.1016/j.snb.2022.132123-
dc.identifier.scopus2-s2.0-85131441605-
dc.identifier.volume367en_US
dc.identifier.spage132123en_US
dc.subject.keywordsGram-Negative Bacteria Detectionen_US
dc.subject.keywordsThermoswitchable Liposomesen_US
dc.description.acknowledgementThis work is supported by the Ministry of Education, Singapore, under its Academic Research Fund Tier 1 (No. RT15/19, to C.L.K.L. and No. RG6/20, to B.X.) and the Agency for Science, Technology and Research, Science and Engineering Research Council (A*Star SERC), Singapore (Nos. A1983c0028 and A20E5c0090, to B.X.).en_US
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item.grantfulltextnone-
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