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https://hdl.handle.net/10356/162040
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Tan, Jie Ren | en_US |
dc.contributor.author | Ferdinandus | en_US |
dc.contributor.author | Xing, Bengang | en_US |
dc.contributor.author | Lee, Ken Chi-Lik | en_US |
dc.date.accessioned | 2022-10-03T01:04:26Z | - |
dc.date.available | 2022-10-03T01:04:26Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Tan, J. R., Ferdinandus, Xing, B. & Lee, K. C. (2022). Functionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteria. Sensors and Actuators B: Chemical, 367, 132123-. https://dx.doi.org/10.1016/j.snb.2022.132123 | en_US |
dc.identifier.issn | 0925-4005 | en_US |
dc.identifier.uri | https://hdl.handle.net/10356/162040 | - |
dc.description.abstract | Biosensors for rapid and sensitive detection of pathogens play a crucial role in healthcare and food safety. Despite the development of numerous sensing devices, it remains a challenge to obtain biosensors that are reliable, easy-to-use and can offer results within minutes. Herein, we report a functionalized liposome, with an embedded fluorescent dye to offer thermoswitchable emission, for selective Gram-negative bacteria detection. This targeting system utilizes polymyxin B (PmB) as a recognition element, where it can be conjugated onto the surface of the liposome to differentiate Gram-negative bacteria from Gram-positive counterparts as well as mammalian cells via fluorescence spectroscopy. The unique thermoswitchable fluorescence conferred through manipulation of aggregation-induced quenching allows for high signal-to-noise detection, reaching up to 3.5-fold increase in emission in the case of E. coli. This is in contrast to typical small organic fluorescent labels and future work includes developing a suitable capture probe to achieve lower limits of detection. | en_US |
dc.description.sponsorship | Agency for Science, Technology and Research (A*STAR) | en_US |
dc.description.sponsorship | Ministry of Education (MOE) | en_US |
dc.language.iso | en | en_US |
dc.relation | RT15/19 | en_US |
dc.relation | RG6/20 | en_US |
dc.relation | A1983c0028 | en_US |
dc.relation | A20E5c0090 | en_US |
dc.relation.ispartof | Sensors and Actuators B: Chemical | en_US |
dc.rights | © 2022 Elsevier B.V. All rights reserved. | en_US |
dc.subject | Science::Chemistry | en_US |
dc.title | Functionalization of thermoswitchable liposomes for rapid detection of Gram-negative bacteria | en_US |
dc.type | Journal Article | en |
dc.contributor.school | School of Physical and Mathematical Sciences | en_US |
dc.identifier.doi | 10.1016/j.snb.2022.132123 | - |
dc.identifier.scopus | 2-s2.0-85131441605 | - |
dc.identifier.volume | 367 | en_US |
dc.identifier.spage | 132123 | en_US |
dc.subject.keywords | Gram-Negative Bacteria Detection | en_US |
dc.subject.keywords | Thermoswitchable Liposomes | en_US |
dc.description.acknowledgement | This work is supported by the Ministry of Education, Singapore, under its Academic Research Fund Tier 1 (No. RT15/19, to C.L.K.L. and No. RG6/20, to B.X.) and the Agency for Science, Technology and Research, Science and Engineering Research Council (A*Star SERC), Singapore (Nos. A1983c0028 and A20E5c0090, to B.X.). | en_US |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
Appears in Collections: | SPMS Journal Articles |
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