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Title: Chemiluminescent probes with long-lasting high brightness for in vivo imaging of neutrophils
Authors: Huang, Jingsheng
Cheng, Penghui
Xu, Cheng
Liew, Si Si
He, Shasha
Zhang, Yan
Pu, Kanyi
Keywords: Engineering::Bioengineering
Issue Date: 2022
Source: Huang, J., Cheng, P., Xu, C., Liew, S. S., He, S., Zhang, Y. & Pu, K. (2022). Chemiluminescent probes with long-lasting high brightness for in vivo imaging of neutrophils. Angewandte Chemie (International Ed. in English), 61(30), e202203235-.
Project: 2019-T1-002-045 
SERC A18A8b0059
Journal: Angewandte Chemie (International ed. in English)
Abstract: Real-time optical imaging of immune cells can contribute to understanding their pathophysiological roles, which still remains challenging. Current sensitive chemiluminophores have issues of short half-lives and low brightness, limiting their ability for in vivo longitudinal monitoring of immunological processes. To tackle these issues, we report benzoazole-phenoxyl-dioxetane (BAPD)-based chemiluminophores with intramolecular hydrogen bonding for in vivo imaging of neutrophils. Compared with the classical counterpart, chemiluminescence half-lives and brightness of BAPDs in the aqueous solution are increased by ∼ 33- and 8.2-fold, respectively. Based on the BAPD scaffold, a neutrophil elastase-responsive chemiluminescent probe is developed for real-time imaging of neutrophils in peritonitis and psoriasis mouse models. Our study provides an intramolecular hydrogen bonding molecular design for improving the performance of chemiluminophores in advanced imaging applications.
ISSN: 1433-7851
DOI: 10.1002/anie.202203235
Rights: © 2022 Wiley-VCH GmbH. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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