Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/162361
Title: Scar prevention through topical delivery of gelatin-tyramine-siSPARC nanoplex loaded in dissolvable hyaluronic acid microneedle patch across skin barrier
Authors: Chun, Yong Yao
Tan, William Wei Ren
Vos, Marcus Ivan Gerard
Chan, Wen Kiat
Tey, Hong Liang
Tan, Nguan Soon
Tan, Timothy Thatt Yang
Keywords: Science::Medicine
Issue Date: 2022
Source: Chun, Y. Y., Tan, W. W. R., Vos, M. I. G., Chan, W. K., Tey, H. L., Tan, N. S. & Tan, T. T. Y. (2022). Scar prevention through topical delivery of gelatin-tyramine-siSPARC nanoplex loaded in dissolvable hyaluronic acid microneedle patch across skin barrier. Biomaterials Science, 10(14), 3963-3971. https://dx.doi.org/10.1039/d2bm00572g
Project: NGF-2019-07-004
CSAINV20nov-0003
Journal: Biomaterials Science
Abstract: Currently, there is no effective method to prevent the formation of hypertrophic scars and keloids, which can cause severe physical and psychological burdens to patients. Secreted protein acidic and cysteine-rich (SPARC) is involved in wound fibrosis by modulating fibroblast functions, causing excessive collagen deposition during wound healing. Thus, the reduction in SPARC gene expression after wounding can contribute to the downstream reduction in collagen production at the wound site and prevent scar formation. In this study, a dissolvable and biocompatible hyaluronic acid (HA) microneedle patch loaded with nanoplexes containing tyramine-modified gelatin and siRNA for SPARC (siSPARC/Gtn-Tyr) was investigated for topical scar prevention. Tyramine-modified gelatin (Gtn-Tyr) provides electrostatic protection and enhances cell internalization for siSPARC. In vitro studies using human dermal fibroblasts showed that both siSPARC/Gtn-Tyr nanoplexes and siSPARC/Gtn-Tyr-loaded microneedle patches can significantly reduce SPARC gene expression (P < 0.05) and do not cause discernable cytotoxic effects. Further studies using a mouse wound model demonstrate that the siSPARC/Gtn-Tyr-loaded microneedle patch can reduce collagen production during wound healing without triggering an immune response. When Gtn-Tyr-siSPARC is administered transdermally at the wound site, effective collagen reduction is achieved through silencing of the matricellular SPARC protein, thus promising the reduction of scar formation. Overall, the siSPARC/Gtn-Tyr loaded microneedle patch can potentially provide an effective transdermal anti-fibrotic treatment.
URI: https://hdl.handle.net/10356/162361
ISSN: 2047-4830
DOI: 10.1039/d2bm00572g
Rights: © 2022 The Royal Society of Chemistry. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles
SCBE Journal Articles

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