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Title: B-box containing proteins mediate seed oil accumulation in Arabidopsis
Authors: Noor Aqilah Noor Azlan
Keywords: Science::Biological sciences::Botany
Issue Date: 2022
Publisher: Nanyang Technological University
Source: Noor Aqilah Noor Azlan (2022). B-box containing proteins mediate seed oil accumulation in Arabidopsis. Master's thesis, Nanyang Technological University, Singapore.
Abstract: WRINKLED1 (WRI1) transcription factor plays a pivotal role in governing plant oil biosynthesis. However, the regulatory mechanism of WRI1 remains unclear. Here, we identify a member from the B-box (BBX) family as a novel interacting partner of Arabidopsis WRI1 (AtWRI1). Currently, there is no evidence showing the involvement of BBX in plant oil metabolism. This project aims to investigate the role of WRI1-BBX module in mediating plant oil biosynthesis. AtWRI1 and BBX physically interacted in the yeast-two-hybrid and the bimolecular fluorescence complementation assays. Seeds of bbx null mutants displayed reduced oil content. In contrary, transgenic lines overexpressing BBX showed increased seed oil content. Co-expression of BBX and AtWRI1 resulted in enhanced transcriptional activity of AtWRI1. Taken together, our preliminary data suggest that BBX might function as a positive regulator of AtWRI1-mediated seed oil accumulation and potentially bridge environmental signals with lipid metabolism.
DOI: 10.32657/10356/162481
Schools: School of Biological Sciences 
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: embargo_20241025
Fulltext Availability: With Fulltext
Appears in Collections:SBS Theses

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