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Title: Discrepant serological findings in SARS-CoV-2 PCR-negative hospitalized patients with fever and acute respiratory symptoms during the pandemic
Authors: Cross, Gail B.
Naftalin, Claire M.
Ngiam, Jinghao N.
Bagdasarian, Natasha
Poh, Chek M.
Goh, Yun S.
Chia, Wan N.
Amrun, Siti N.
Tham, Sai M.
Teng, Hazel
Alagha, Rawan
Kumar, Shoban K.
Tan, Shaun S. Y.
Wang, Lin F.
Tambyah, Paul A.
Renia, Laurent
Fisher, Dale
Ng, Lisa F. P.
Keywords: Science::Medicine
Issue Date: 2022
Source: Cross, G. B., Naftalin, C. M., Ngiam, J. N., Bagdasarian, N., Poh, C. M., Goh, Y. S., Chia, W. N., Amrun, S. N., Tham, S. M., Teng, H., Alagha, R., Kumar, S. K., Tan, S. S. Y., Wang, L. F., Tambyah, P. A., Renia, L., Fisher, D. & Ng, L. F. P. (2022). Discrepant serological findings in SARS-CoV-2 PCR-negative hospitalized patients with fever and acute respiratory symptoms during the pandemic. Journal of Medical Virology, 94(6), 2460-2470.
Journal: Journal of Medical Virology
Abstract: Coronavirus Disease 2019 (COVID-19) serology has an evolving role in the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. However, its use in hospitalized patients with acute respiratory symptoms remains unclear. Hospitalized patients with acute respiratory illness admitted to an isolation ward were recruited. All patients had negative nasopharyngeal swab polymerase chain reaction (PCR) for SARS-CoV-2. Serological studies using four separate assays (cPass: surrogate neutralizing enzyme-linked immunosorbent assay [ELISA]; Elecsys: N-antigen based chemiluminescent assay; SFB: S protein flow-based; epitope peptide-based ELISA) were performed on stored plasma collected from patients during the initial hospital stay, and a convalescent visit 4-12 weeks later. Of the 51 patients studied (aged 54, interquartile range 21-84; 62.7% male), no patients tested positive on the Elecsys or cPass assays. Out of 51 patients, 5 had antibodies detected on B-cell Epitope Assay and 3/51 had antibodies detected on SFB assay. These 8 patients with positive serological test to COVID-19 were more likely to have a high-risk occupation (p = 0.039), bacterial infection (p = 0.028), and neutrophilia (p = 0.013) during their initial hospital admission. Discrepant COVID-19 serological findings were observed among those with recent hospital admissions and bacterial infections. The positive serological findings within our cohort raise important questions about the interpretation of sero-epidemiology during the current pandemic.
ISSN: 0146-6615
DOI: 10.1002/jmv.27656
Rights: © 2022 Wiley Periodicals LLC. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:LKCMedicine Journal Articles
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