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Title: Population-based variations of a core resistome revealed by urban sewage metagenome surveillance
Authors: Li, Wenxuan
Mao, Feijian
Ng, Charmaine
Jong, Mui Choo
Goh, Shin Giek
Charles, Francis Rathinam
Ng, Oon Tek
Marimuthu, Kalisvar
He, Yiliang
Gin, Karina Yew-Hoong
Keywords: Science::Medicine
Issue Date: 2022
Source: Li, W., Mao, F., Ng, C., Jong, M. C., Goh, S. G., Charles, F. R., Ng, O. T., Marimuthu, K., He, Y. & Gin, K. Y. (2022). Population-based variations of a core resistome revealed by urban sewage metagenome surveillance. Environment International, 163, 107185-.
Project: MOH-000276 
Journal: Environment International 
Abstract: Sewage-based surveillance is widely employed to understand the occurrence and distribution of antimicrobial resistance (AMR) in urban community. However, there are limited studies which investigated the sewage of different sources within community. The present study used metagenomics to decipher the AMR profiles in five sources: local residence's source, animal source, migrant workers' source, clinical source , and urban wastewater treatment plant influent. A core resistome of ARGs was found across all samples, accounting for 81.4%-93.3% of the abundance of total resistome with only 17.3% diversity, irrespective of the sewage sources. Clinically relevant ARGs were identified in the core resistome across all wastewater sources. This included genes conferring resistance to beta-lactams as biomarkers of hospital sewage. The pet center wastewater showed a high abundance of genes encoding resistance to tetracycline, which is a commonly used veterinary antibiotic. The resistome profile of sewage from the migrant workers' dormitories showed a slight variation to that of the local residential population, suggesting possible differences in the human gut resistome of the foreign/migrant population, with biomarkers of genes encoding resistance to fosfomycin, fosmidomycin, kasugamycin, MLS, and polymyxin. The co-localization of ARGs and plasmid, MGEs and integrative and conjugative elements (ICEs) could explain variations in the core resistome, presumably a result of high antibiotic selection pressure. Further analysis showed a specific host-associated resistance pattern, in which core hosts mediated the core resistome profile. The core BMRGs were also co-localized with MGEs/ICEs and carried by core potential bacterial hosts. Local healthy population carried the lowest ARG load (copy number discharged by each person per day) but contributed the highest ARG burden (copy number discharged by the population). This study elucidates population-based variations of a core resistome, and further provides important insights into source tracking and management of AMR in urban environments.
ISSN: 0160-4120
DOI: 10.1016/j.envint.2022.107185
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: National Centre for Infectious Diseases, Singapore
Tan Tock Seng Hospital
Rights: © 2022 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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