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Title: Self-assembly of oriented antibody-decorated metal-organic framework nanocrystals for active-targeting applications
Authors: Alt, Karen
Carraro, Francesco
Jap, Edwina
Linares-Moreau, Mercedes
Riccò, Raffaele
Righetto, Marcello
Bogar, Marco
Amenitsch, Heinz
Hashad, Rania A.
Doonan, Christian
Hagemeyer, Christoph E.
Falcaro, Paolo
Keywords: Science::Physics
Issue Date: 2022
Source: Alt, K., Carraro, F., Jap, E., Linares-Moreau, M., Riccò, R., Righetto, M., Bogar, M., Amenitsch, H., Hashad, R. A., Doonan, C., Hagemeyer, C. E. & Falcaro, P. (2022). Self-assembly of oriented antibody-decorated metal-organic framework nanocrystals for active-targeting applications. Advanced Materials, 34(21), e2106607-.
Journal: Advanced Materials 
Abstract: Antibody (Ab)-targeted nanoparticles are becoming increasingly important for precision medicine. By controlling the Ab orientation, targeting properties can be enhanced; however, to afford such an ordered configuration, cumbersome chemical functionalization protocols are usually required. This aspect limits the progress of Abs-nanoparticles toward nanomedicine translation. Herein, a novel one-step synthesis of oriented monoclonal Ab-decorated metal-organic framework (MOF) nanocrystals is presented. The crystallization of a zinc-based MOF, Zn2 (mIM)2 (CO3 ), from a solution of Zn2+ and 2-methylimidazole (mIM), is triggered by the fragment crystallizable (Fc) region of the Ab. This selective growth yields biocomposites with oriented Abs on the MOF nanocrystals (MOF*Ab): the Fc regions are partially inserted within the MOF surface and the antibody-binding regions protrude from the MOF surface toward the target. This ordered configuration imparts antibody-antigen recognition properties to the biocomposite and shows preserved target binding when compared to the parental antibodies. Next, the biosensing performance of the system is tested by loading MOF*Ab with luminescent quantum dots (QD). The targeting efficiency of the QD-containing MOF*Ab is again, fully preserved. The present work represents a simple self-assembly approach for the fabrication of antibody-decorated MOF nanocrystals with broad potential for sensing, diagnostic imaging, and targeted drug delivery.
ISSN: 0935-9648
DOI: 10.1002/adma.202106607
Schools: School of Physical and Mathematical Sciences 
Rights: © 2022 The Authors. Advanced Materials published by Wiley-VCH GmbH. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and repro-duction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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