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Title: Cyanine-dyad molecular probe for the simultaneous profiling of the evolution of multiple radical species during bacterial infections
Authors: Wang, Zhimin
Cong, Thang Do
Zhong, Wenbin
Lau, Jun Wei
Kwek, Germain
Chan-Park, Mary B.
Xing, Bengang
Keywords: Science::Chemistry
Issue Date: 2021
Source: Wang, Z., Cong, T. D., Zhong, W., Lau, J. W., Kwek, G., Chan-Park, M. B. & Xing, B. (2021). Cyanine-dyad molecular probe for the simultaneous profiling of the evolution of multiple radical species during bacterial infections. Angewandte Chemie International Edition, 60(31), 16900-16905.
Project: RG6/20
MOE 2017-T2-2-11
Journal: Angewandte Chemie International Edition 
Abstract: Real-time monitoring of the evolution of bacterial infection-associated multiple radical species is critical to accurately profile the pathogenesis and host-defense mechanisms. Here, we present a unique dual wavelength near-infrared (NIR) cyanine-dyad molecular probe (HCy5-Cy7) for simultaneous monitoring of reactive oxygen and nitrogen species (RONS) variations both in vitro and in vivo. HCy5-Cy7 specifically turns on its fluorescence at 660 nm via superoxide or hydroxyl radical (O2 .- , . OH)-mediated oxidation of reduced HCy5 moiety to Cy5, while peroxynitrite or hypochlorous species (ONOO- , ClO- )-induced Cy7 structural degradation causes the emission turn-off at 800 nm. Such multispectral but reverse signal responses allow multiplex manifestation of in situ oxidative and nitrosative stress events during the pathogenic and defensive processes in both bacteria-infected macrophage cells and living mice. Most importantly, this study may also provide new perspectives for understanding the bacterial pathogenesis and advancing the precision medicine against infectious diseases.
ISSN: 1433-7851
DOI: 10.1002/anie.202104100
Rights: © 2021 Wiley-VCH GmbH. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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