Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/162856
Title: Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes
Authors: Suntornnond, Ratima
Ng, Wei Long
Huang, Xi
Yeow, Ethan Chuen Herh
Yeong, Wai Yee
Keywords: Engineering::Materials
Issue Date: 2022
Source: Suntornnond, R., Ng, W. L., Huang, X., Yeow, E. C. H. & Yeong, W. Y. (2022). Improving printability of hydrogel-based bio-inks for thermal inkjet bioprinting applications via saponification and heat treatment processes. Journal of Materials Chemistry B, 10(31), 5989-6000. https://dx.doi.org/10.1039/d2tb00442a
Project: IAF-ICP 
Journal: Journal of Materials Chemistry B 
Abstract: Material jetting bioprinting is a highly promising three-dimensional (3D) bioprinting technique that facilitates drop-on-demand (DOD) deposition of biomaterials and cells at pre-defined positions with high precision and resolution. A major challenge that hinders the prevalent use of the material jetting bioprinting technique is due to its limited range of printable hydrogel-based bio-inks. As a proof-of-concept, further modifications were made to gelatin methacrylate (GelMA), a gold-standard bio-ink, to improve its printability in a thermal inkjet bioprinter (HP Inc. D300e Digital Dispenser). A two-step modification process comprising saponification and heat treatment was performed; the GelMA bio-ink was first modified via a saponification process under highly alkali conditions to obtain saponified GelMA (SP-GelMA), followed by heat treatment via an autoclaving process to obtain heat-treated SP-GelMA (HSP-GelMA). The bio-ink modification process was optimized by evaluating the material properties of the GelMA bio-inks via rheological characterization, the bio-ink crosslinking test, nuclear magnetic resonance (NMR) spectroscopy and the material swelling ratio after different numbers of heat treatment cycles (0, 1, 2 and 3 cycles). Lastly, size-exclusion chromatography with multi-angle light scattering (SEC-MALS) was performed to determine the effect of heat treatment on the molecular weight of the bio-inks. In this work, the 4% H2SP-GelMA bio-inks (after 2 heat treatment cycles) demonstrated good printability and biocompatibility (in terms of cell viability and proliferation profile). Furthermore, thermal inkjet bioprinting of the modified hydrogel-based bio-ink (a two-step modification process comprising saponification and heat treatment) via direct/indirect cell patterning is a facile approach for potential fundamental cell-cell and cell-material interaction studies.
URI: https://hdl.handle.net/10356/162856
ISSN: 2050-750X
DOI: 10.1039/d2tb00442a
Rights: © 2022 The Royal Society of Chemistry. All rights reserved. This paper was published in Journal of Materials Chemistry B and is made available with permission of The Royal Society of Chemistry.
Fulltext Permission: embargo_20230722
Fulltext Availability: With Fulltext
Appears in Collections:MAE Journal Articles
SC3DP Journal Articles

Files in This Item:
File Description SizeFormat 
Improving printability of hydrogel based bio inks for thermal inkjet bioprinting applications via saponification and heat treatment processes.pdf
  Until 2023-07-22
1.67 MBAdobe PDFUnder embargo until Jul 22, 2023

SCOPUSTM   
Citations 50

1
Updated on Nov 26, 2022

Web of ScienceTM
Citations 50

2
Updated on Nov 28, 2022

Page view(s)

16
Updated on Nov 30, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.