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|Title:||Directed evolution of phage lysins||Authors:||Cheng, Xin Yi||Keywords:||Science::Biological sciences||Issue Date:||2022||Publisher:||Nanyang Technological University||Source:||Cheng, X. Y. (2022). Directed evolution of phage lysins. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/162982||Project:||SBS22-058||Abstract:||Resistance to carbapenem, a ‘last resort’ antibiotic, has been discovered in two major nosocomial pathogens, Acinetobacter baumannii and Pseudomonas aeruginosa. The dearth of novel antibiotics being developed necessitates alternative antimicrobial approaches. Lysins are promising alternative antimicrobial agents due to their rapid bactericidal activity and low likelihood of resistance development. However, Gram-negative lysins are inhibited under physiologically relevant conditions such as in the presence of human serum/NaCl. Directed evolution may be employed to improve the activity and overcome the limitations of lysins. Though, the present lysin library screening procedure is labour-intensive and time-consuming. Therefore, we developed and validated a novel high-throughput lysin screening method. This method was effective in screening lysins activity. Additionally, we have generated a diverse library of lysin using error-prone PCR. We propose adapting this high-throughput lysin screening method to expedite the discovery of improved variants in lysin libraries.||URI:||https://hdl.handle.net/10356/162982||Schools:||School of Biological Sciences||Research Centres:||NTU Institute of Structural Biology||Fulltext Permission:||embargo_restricted_20241115||Fulltext Availability:||With Fulltext|
|Appears in Collections:||SBS Student Reports (FYP/IA/PA/PI)|
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|FYP Thesis_Cheng Xin Yi_Final.pdf|
|722.44 kB||Adobe PDF||Under embargo until Nov 15, 2024|
Updated on Dec 8, 2023
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