Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/162994
Title: Human hair proteins as natural reactive oxygen species scavengers for in vitro applications
Authors: Lai, Hui Ying
Setyawati, Magdiel Inggrid
Duarte, Catarina Vizetto
Chua, Huei Min
Low, Choon Teck
Ng, Kee Woei
Keywords: Engineering::Materials
Engineering::Materials::Biomaterials
Issue Date: 2022
Source: Lai, H. Y., Setyawati, M. I., Duarte, C. V., Chua, H. M., Low, C. T. & Ng, K. W. (2022). Human hair proteins as natural reactive oxygen species scavengers for in vitro applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials. https://dx.doi.org/10.1002/jbm.b.35203
Project: IAF-PP H17/01/a0/0L9 
RT10/20 
Journal: Journal of Biomedical Materials Research Part B: Applied Biomaterials 
Abstract: Human hair proteins are recognized for their intrinsically high cysteine content. They can be solubilized while preserving their highly reductive thiol groups for free radical scavenging applications. The presence of aromatic and nucleophilic amino acids such as methionine, serine, phenylalanine and threonine further contribute to the antioxidative potential of this material. Herein, utilizing the DPPH (2,2-diphenyl-1-picrylhydrazyl) and acellular 2',7'- dichlorodihydrofluorescein diacetate (H2DCFDA) assays, keratins are demonstrated to possess the highest radical scavenging activity among the studied hair proteins. Consequently, protection against hydrogen peroxide-induced oxidative stress in Human Dermal Fibroblasts (HDF) cultured in human hair keratin supplemented media is demonstrated. Quenching of reactive oxygen species(ROS) in the HDF is observed using the CellROX Green dye and the expression levels of antioxidant (HMOX1, SOD2, GPX1) and tumor suppressor (TP53) genes is analyzed using qPCR. Collectively, this study presents further evidence and demonstrates the in vitro application potential of hair proteins, especially keratins, as an antioxidizing supplement.
URI: https://hdl.handle.net/10356/162994
ISSN: 1552-4973
DOI: 10.1002/jbm.b.35203
Rights: © 2022 Wiley Periodicals LLC. This is the peer reviewed version of the following article: Lai, H. Y., Setyawati, M. I., Duarte, C. V., Chua, H. M., Low, C. T. & Ng, K. W. (2022). Human hair proteins as natural reactive oxygen species scavengers for in vitro applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, which has been published in final form at https://doi.org/10.1002/jbm.b.35203. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
Fulltext Permission: embargo_20231130
Fulltext Availability: With Fulltext
Appears in Collections:IGS Journal Articles
MSE Journal Articles
NEWRI Journal Articles

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