Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/163083
Title: Time-restricted feeding modulates the DNA methylation landscape, attenuates hallmark neuropathology and cognitive impairment in a mouse model of vascular dementia
Authors: Selvaraji, Sharmelee
Efthymios, Motakis
Foo, Roger Sik Yin
Fann, David Y.
Lai, Mitchell Kim Peng
Chen, Christopher Li Hsian
Lim, Kah-Leong
Arumugam, Thiruma V.
Keywords: Science::Medicine
Issue Date: 2022
Source: Selvaraji, S., Efthymios, M., Foo, R. S. Y., Fann, D. Y., Lai, M. K. P., Chen, C. L. H., Lim, K. & Arumugam, T. V. (2022). Time-restricted feeding modulates the DNA methylation landscape, attenuates hallmark neuropathology and cognitive impairment in a mouse model of vascular dementia. Theranostics, 12(7), 3007-3023. https://dx.doi.org/10.7150/thno.71815
Project: NMRC-CBRG-0102/2016 
NMRC/CSA-SI/007/2016 
NMRC/OFIRG/ 0036/2017 
MOE2017-T3-1-002 
Journal: Theranostics 
Abstract: Objective: Vascular dementia (VaD) is the second most common cause of dementia worldwide. The increasing contribution of lifestyle-associated risk factors to VaD has pointed towards gene-environment interactions (i.e. epigenetics). This study thus aims to investigate the DNA methylation landscape in a chronic cerebral hypoperfusion (CCH) mouse model of VaD. As a nexus between the gene-environment interaction, intermittent fasting (IF) was introduced as a prophylactic intervention. Methods: Bilateral common carotid artery stenosis (BCAS) was used to induce CCH by placing micro-coils of 0.18 mm in each common carotid artery of the mice. The coils were left in the mice for 7, 15 and 30 days to study temporal differences. IF was introduced for 16 h daily for 4 months prior to BCAS. Reduced Representation Bisulfite Sequencing (RRBS) was used to study the DNA methylation landscape. Cognitive impairment was measured using Barnes Maze Test. White matter lesions (WML) and neuronal loss were measured using Luxol fast blue staining and cresyl violet staining respectively. Results: IF mice subjected to CCH displayed significantly better cognitive learning ability and memory, improved neuropathological alterations with reduced WMLs and neuronal loss. Modulation of DNA methylation patterns in the cortex of AL CCH mice was re-modelled and signs of reversal was observed in IF CCH mice across all three timepoints. Conclusions: These findings provide an understanding of how IF may protect the brain against damage caused by CCH and show promise in offering potential beneficial effects in mitigating the neuropathology and cognitive deficits in VaD.
URI: https://hdl.handle.net/10356/163083
ISSN: 1838-7640
DOI: 10.7150/thno.71815
Rights: © The author(s). This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

Files in This Item:
File Description SizeFormat 
thnov12p3007.pdf3.05 MBAdobe PDFView/Open

SCOPUSTM   
Citations 50

2
Updated on Nov 29, 2022

Web of ScienceTM
Citations 50

2
Updated on Dec 6, 2022

Page view(s)

14
Updated on Dec 8, 2022

Download(s)

1
Updated on Dec 8, 2022

Google ScholarTM

Check

Altmetric


Plumx

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.