Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/163095
Title: Insights into POT1 structural dynamics revealed by cryo-EM
Authors: Smith, Emmanuel W.
Lattmann, Simon
Liu, Barry Zhehui
Ahsan, Bilal
Rhodes, Daniela
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Smith, E. W., Lattmann, S., Liu, B. Z., Ahsan, B. & Rhodes, D. (2022). Insights into POT1 structural dynamics revealed by cryo-EM. PloS One, 17(2), e0264073-. https://dx.doi.org/10.1371/journal.pone.0264073
Project: MOE2012-T3-1- 001
Journal: PloS one
Abstract: Telomeres are protein-DNA complexes that protect the ends of linear eukaryotic chromosomes. Mammalian telomeric DNA consists of 5'-(TTAGGG)n-3' double-stranded repeats, followed by up to several hundred bases of a 3' single-stranded G-rich overhang. The G-rich overhang is bound by the shelterin component POT1 which interacts with TPP1, the component involved in telomerase recruitment. A previously published crystal structure of the POT1 N-terminal half bound to the high affinity telomeric ligand 5'-TTAGGGTTAG-3' showed that the first six nucleotides, TTAGGG, are bound by the OB1 fold, while the adjacent OB2 binds the last four, TTAG. Here, we report two cryo-EM structures of full-length POT1 bound by the POT1-binding domain of TPP1. The structures differ in the relative orientation of the POT1 OB1 and OB2, suggesting that these two DNA-binding OB folds take up alternative conformations. Supporting DNA binding studies using telomeric ligands in which the OB1 and OB2 binding sites were spaced apart, show that POT1 binds with similar affinities to spaced or contiguous binding sites, suggesting plasticity in DNA binding and a role for the alternative conformations observed. A likely explanation is that the structural flexibility of POT1 enhances binding to the tandemly arranged telomeric repeats and hence increases telomere protection.
URI: https://hdl.handle.net/10356/163095
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0264073
Rights: © 2022 Smith et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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