Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/163105
Title: A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae
Authors: Xie, Ying
Zhou, Feng
Ma, Qianqian
Lu, Lanyuan
Miao, Yansong
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Xie, Y., Zhou, F., Ma, Q., Lu, L. & Miao, Y. (2022). A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae. Molecular Biology of the Cell, 33(2), ar19-. https://dx.doi.org/10.1091/mbc.E21-06-0285
Project: M4081533 
SRIS, SIG18002
MOE2016-T2-1-005
MOE2019-T3-1-012 
2018-T1-001-096
Journal: Molecular Biology of the Cell
Abstract: Actin nucleation is achieved by collaborative teamwork of actin nucleator factors (NFs) and nucleation-promoting factors (NPFs) into functional protein complexes. Selective inter- and intramolecular interactions between the nucleation complex constituents enable diverse modes of complex assembly in initiating actin polymerization on demand. Budding yeast has two formins, Bni1 and Bnr1, which are teamed up with different NPFs. However, the selective pairing between formin NFs and NPFs into the nucleation core for actin polymerization is not completely understood. By examining the functions and interactions of NPFs and NFs via biochemistry, genetics, and mathematical modeling approaches, we found that two NPFs, Aip5 and Bud6, showed joint teamwork effort with Bni1 and Bnr1, respectively, by interacting with the C-terminal intrinsically disordered region (IDR) of formin, in which two NPFs work together to promote formin-mediated actin nucleation. Although the C-terminal IDRs of Bni1 and Bnr1 are distinct in length, each formin IDR orchestrates the recruitment of Bud6 and Aip5 cooperatively by different positioning strategies to form a functional complex. Our study demonstrated the dynamic assembly of the actin nucleation complex by recruiting multiple partners in budding yeast, which may be a general feature for effective actin nucleation by formins.
URI: https://hdl.handle.net/10356/163105
ISSN: 1939-4586
DOI: 10.1091/mbc.E21-06-0285
Rights: © 2022 Xie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/4.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
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