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dc.contributor.authorLim, Carey Fang Huien_US
dc.identifier.citationLim, C. F. H. (2022). Ionic regulation of mycobacterial infection by potassium and potassium channel. Doctoral thesis, Nanyang Technological University, Singapore.
dc.description.abstractEpigenetic analysis of monocytes from active TB patients demonstrated enrichment of KCNJ15, which encodes an inwardly rectifying K+ channel (Kir4.2). We investigated the function of Kir4.2 in mycobacterial infection using a gain and loss-of-function approach and knockout mouse model. Kir4.2 was observed to promote inflammation and apoptosis and led to changes in immune populations in the lung and their ability to control bacterial burden. Additionally, we found that the necrotic mycobacterial granuloma microenvironment possessed a high K+ milieu. Exploring the effects of high extracellular K+ ([K+]e) on monocytes revealed that K+ influx resulted in mitochondrial dysfunction-induced autophagic bacterial killing. Thus, this study investigated the role of KCNJ15/Kir4.2 and high [K+]e on the acute host immune response against mycobacterial infection, which may inform future potassium-targeting host-directed therapeutics.en_US
dc.publisherNanyang Technological Universityen_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).en_US
dc.subjectScience::Biological sciences::Microbiology::Immunologyen_US
dc.titleIonic regulation of mycobacterial infection by potassium and potassium channelen_US
dc.typeThesis-Doctor of Philosophyen_US
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.description.degreeDoctor of Philosophyen_US
dc.contributor.organizationA*STAR Infectious Diseases Labsen_US
dc.contributor.supervisor2Amit Singhalen_US
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