Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/16319
Title: Spatial organization of synaptic plasticity in cerebellar parallel fiber : Purkinje cell synapses
Authors: Zhou, Ariyo Mitriya Wenyong
Keywords: DRNTU::Science::Biological sciences::Cytology
Issue Date: 2009
Abstract: Not much is known about spatial patterns of Parallel fiber-Purkinje cell long-term depression (PF-PC LTD). For this reason, I visualized the distribution of PF-PC LTD in relation to the area of conjunctive stimulation by using voltage-sensitive dye imaging. During stimulation, the basic optical response was observed to have an early “spiky” component and a slower trailing component. Following a 1 Hz train of stimuli, LTD was induced and could be visualized as a decrease in the response elicited by subsequent stimuli. Such LTD was restricted to the molecular layer of the cerebellum, consistent with a decrease in transmission at the PF-PC synapse. Ratio imaging allowed me to further characterize the spatial range of LTD. Line scan analysis revealed that the amount of LTD generally decreased with distance from the stimulation site, demonstrating a gradient limited by the distribution of activated PF synapses. In addition, LTD extended for 80 μm further than the peak area of PF activation, consistent with previously indications of the spread of LTD beyond active PF synapses. I conclude that LTD spread is likely to be limited by 2 things: (1) the distribution of activated PF synapses; and (2) the spread of chemical signals generated during LTD.
URI: http://hdl.handle.net/10356/16319
Rights: Nanyang Technological University
Fulltext Permission: restricted
Fulltext Availability: With Fulltext
Appears in Collections:SBS Student Reports (FYP/IA/PA/PI)

Files in This Item:
File Description SizeFormat 
Ariyo_THESIS.pdf
  Restricted Access
3.82 MBAdobe PDFView/Open

Page view(s) 50

316
checked on Oct 1, 2020

Download(s) 50

6
checked on Oct 1, 2020

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.