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Title: Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity
Authors: Ethiraj, Lalith Prabha
Fong, Samuel En Lei
Liu, Ranran
Chan, Madelynn
Winkler, Christoph
Carney, Tom James
Keywords: Science::Medicine
Issue Date: 2022
Source: Ethiraj, L. P., Fong, S. E. L., Liu, R., Chan, M., Winkler, C. & Carney, T. J. (2022). Colorimetric and fluorescent TRAP assays for visualising and quantifying fish osteoclast activity. European Journal of Histochemistry, 66(2).
Journal: European Journal of Histochemistry 
Abstract: Histochemical detection of tartrate-resistant acid phosphatase (TRAP) activity is a fundamental technique for visualizing osteoclastic bone resorption and assessing osteoclast activity status in tissues. This approach has mostly employed colorimetric detection, which has limited quantification of activity in situ and co-labelling with other skeletal markers. Here we report simple colorimetric and fluorescent TRAP assays in zebrafish and medaka, two important model organisms for investigating the pathogenesis of bone disorders. We show fluorescent TRAP staining, utilising the ELF97 substrate, is a rapid, robust and stable system to visualise and quantify osteoclast activity in zebrafish, and is compatible with other fluorescence stains, transgenic lines and antibody approaches. Using this approach, we show that TRAP activity is predominantly found around the base of the zebrafish pharyngeal teeth, where osteoclast activity state appears to be heterogeneous.
ISSN: 1121-760X
DOI: 10.4081/ejh.2022.3369
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: Tan Tock Seng Hospital
Institute of Molecular and Cell Biology (IMCB), A*STAR
Rights: © the Author(s), 2022. This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles

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