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Title: Cytoadherence properties of Plasmodium knowlesi-infected erythrocytes
Authors: Lee, Wenn-Chyau
Shahari, Shahhaziq
Nguee, Samantha Yee Teng
Lau, Yee-Ling
Rénia, Laurent
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Lee, W., Shahari, S., Nguee, S. Y. T., Lau, Y. & Rénia, L. (2022). Cytoadherence properties of Plasmodium knowlesi-infected erythrocytes. Frontiers in Microbiology, 12, 804417-.
Project: NMRC/OFYIRG/0070/2018 
JCO-DP BMSI/15-800006-SIGN 
Journal: Frontiers in Microbiology 
Abstract: Plasmodium knowlesi is responsible for zoonotic malaria infections that are potentially fatal. While the severe pathology of falciparum malaria is associated with cytoadherence phenomena by Plasmodium falciparum-infected erythrocytes (IRBC), information regarding cytoadherence properties of P. knowlesi-IRBC remained scarce. Here, we characterized the cytoadherence properties of RBC infected with the laboratory-adapted P. knowlesi A1-H.1 strain. We found that late-stage IRBC formed rosettes in a human serum-dependent manner, and rosettes hampered IRBC phagocytosis. IRBC did not adhere much to unexposed (unstimulated) human endothelial cell lines derived from the brain (hCMEC/D3), lungs (HPMEC), and kidneys (HRGEC). However, after being "primed" with P. knowlesi culture supernatant, the IRBC-endothelial cytoadherence rate increased in HPMEC and HRGEC, but not in hCMEC/D3 cells. Both endothelial cytoadherence and rosetting phenomena were abrogated by treatment of P. knowlesi-IRBC with trypsin. We also found that different receptors were involved in IRBC cytoadherence to different types of endothelial cells. Although some of the host receptors were shared by both P. falciparum- and P. knowlesi-IRBC, the availability of glycoconjugates on the receptors might influence the capacity of P. knowlesi-IRBC to cytoadhere to these receptors.
ISSN: 1664-302X
DOI: 10.3389/fmicb.2021.804417
Schools: Lee Kong Chian School of Medicine (LKCMedicine) 
School of Biological Sciences 
Organisations: A*STAR Infectious Diseases Labs
Rights: © 2022 Lee, Shahari, Nguee, Lau and Rénia. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:LKCMedicine Journal Articles
SBS Journal Articles

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