Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/163357
Title: Designing siRNA/chitosan-methacrylate complex nanolipogel for prolonged gene silencing effects
Authors: Cao, Ye
Tan, Yang Fei
Wong, Yee Shan
Aminuddin, Muhammad
Ramya, Bhuthalingam
Liew, Melvin Wen Jie
Liu, Jiaxin
Venkatraman, Subbu S.
Keywords: Engineering::Materials
Issue Date: 2022
Source: Cao, Y., Tan, Y. F., Wong, Y. S., Aminuddin, M., Ramya, B., Liew, M. W. J., Liu, J. & Venkatraman, S. S. (2022). Designing siRNA/chitosan-methacrylate complex nanolipogel for prolonged gene silencing effects. Scientific Reports, 12(1), 3527-. https://dx.doi.org/10.1038/s41598-022-07554-0
Journal: Scientific Reports 
Abstract: Despite immense revolutionary therapeutics potential, sustaining release of active small interfering RNA (siRNA) remains an arduous challenge. The development of nanoparticles with siRNA sustained release capabilities provides an avenue to enhance the therapeutic efficacy of gene-based therapy. Herein, we present a new system based on the encapsulation of siRNA/chitosan-methacrylate (CMA) complexes into liposomes to form UV crosslinkable Nanolipogels (NLGs) with sustained siRNA-release properties in vitro. We demonstrated that the CMA nanogel in NLGs can enhance the encapsulation efficiency of siRNA and provide sustained release of siRNA up to 28 days. To understand the particle mechanism of cellular entry, multiple endocytic inhibitors have been used to investigate its endocytosis pathways. The study saw positively charged NLGs entering cells via multiple endocytosis pathways, facilitating endosomal escape and slowly releasing siRNA into the cytoplasm. Transfection experiments confirmed that the crosslinked NLG delivery system provides effective transfection and prolonged silencing effect up to 14 days in cell cultures. We expect that this sustained-release siRNA NLG platform would be of interest in both fundamental biological studies and in clinical applications to extend the use of siRNA-based therapies.
URI: https://hdl.handle.net/10356/163357
ISSN: 2045-2322
DOI: 10.1038/s41598-022-07554-0
Rights: © The Author(s) 2022. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Journal Articles

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