Please use this identifier to cite or link to this item:
Title: Biomimetic photonic coatings using squid reflectin protein
Authors: Loke, Jun Jie
Keywords: Engineering::Materials::Biomaterials
Issue Date: 2022
Publisher: Nanyang Technological University
Source: Loke, J. J. (2022). Biomimetic photonic coatings using squid reflectin protein. Doctoral thesis, Nanyang Technological University, Singapore.
Abstract: Squids are masters of camouflage in the Animal Kingdom. They use metachrosis to dynamically control the morphology of dermal cells – chromatophore and iridophore – that regulate body colouration and patterns to convey signals. This is prevalent in Nature and typically serves important survival functions to deter predators, capture prey or for mating strategies Squids in the Loliginidae family (which included Sepioteuthis lessioniana used in this study) possess the unique capability to dynamically modulate the iridescent properties of their skin by tuning and controlling the internal assembly and periodicity of Bragg-like reflector platelets located within iridophores, which are entirely made of proteins called reflectins. These iridescent light reflective-refractive structures rely on Bragg reflectors, making use of periodic spacing of photonic crystals and thin film constructive interference. Previous studies have demonstrated that these photonic changes are regulated by phosphorylation/dephosphorylation of condensed reflectin nanoparticles in the reflector platelets. Since the size of the reflectin nanoparticles affect the iridescence properties, it was hypothesized that the colour of films/coatings made of reflectin nanoparticles could be regulated by changing the size of reflectin nanoparticles that they are made of. Thus, the main goal was to self-assemble reflectin nanoparticles with well-controlled particle size and investigate if Sepioteuthis lessioniana reflectin B1 (SlRF-B1) nanoparticle was able to self-assemble into photonic lattices to produce structural colouration or iridescence. This thesis describes a systematic approach to self-assemble SlRF-B1 into discrete nanoparticle sizes when conjugated with click-chemistry ligand dibenzocyclooctyne (DBCO)-sulfo-NHS ester. This was achieved by varying the solvent conditions during the self-assembly process. These quasi-monodisperse nanoparticles were subsequently immobilised onto azide-functionalised wafer surface using click-chemistry, resulting in monolayer assemblies where its photonic properties were investigated. These DBCO-SlRF-B1 nanoparticles produced reflectance in the visible wavelength when inter-particle spacing was less than 1 μm. The monolayers exhibit a tunable reflectance response from violet (400 nm) to infrared-red (800 nm).
DOI: 10.32657/10356/163521
Schools: School of Materials Science and Engineering 
Research Centres: Biological & Biomimetic Material Laboratory @ NTU 
Rights: This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:MSE Theses

Files in This Item:
File Description SizeFormat 
Loke Jun Jie PhD Thesis.pdf7.27 MBAdobe PDFThumbnail

Page view(s)

Updated on Feb 27, 2024

Download(s) 50

Updated on Feb 27, 2024

Google ScholarTM




Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.