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Title: Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells
Authors: Ferdinandus, Ferdinandus
Tan, Jie Ren
Lim, Jin Heng
Arai, Satoshi
Sou, Keitaro
Lee, Ken Chi-Lik
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Ferdinandus, F., Tan, J. R., Lim, J. H., Arai, S., Sou, K. & Lee, K. C. (2022). Squaraine probes for the bimodal staining of lipid droplets and endoplasmic reticulum imaging in live cells. Analyst, 147(15), 3570-3577.
Project: RT15/19
Journal: Analyst
Abstract: Lipid droplets (LDs) have emerged as a hot target for cancer therapeutics in recent years owing to findings that have shown them to be key organelles involved in maintaining cellular stability and regulating inter-organelle communication through molecular trafficking. LDs emerge from the endoplasmic reticulum (ER) as a form of cellular homeostasis control. We herein report the study of a library of asymmetric squaraines as superior fluorescence probes to track and image LDs in their native state and environment within cancer cells. The probes are highly selective towards LDs and displayed prominent bright fluorescence with just 1 mu M probe concentration. They also possess bimodal LD and ER staining capability via the simple diffusion of small lipophilic molecules. The probes almost instantly stained LDs, while the ER staining rate is dependent on the probe's lipophilicity and the incubation duration. These "on-demand" organelle-selective probes are highly desirable tools for revealing the role of LDs in governing many cellular processes, especially in malignant cells.
ISSN: 0003-2654
DOI: 10.1039/D2AN00803C
Rights: © 2022 The Royal Society of Chemistry. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
Appears in Collections:SPMS Journal Articles

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