Please use this identifier to cite or link to this item:
https://hdl.handle.net/10356/163555
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Li, Ping | en_US |
dc.contributor.author | Jing, Ruxian | en_US |
dc.contributor.author | Zhou, Mengping | en_US |
dc.contributor.author | Jia, Pei | en_US |
dc.contributor.author | Li, Zhuoya | en_US |
dc.contributor.author | Liu, Guosheng | en_US |
dc.contributor.author | Wang, Zhenyu | en_US |
dc.contributor.author | Wang, Hailei | en_US |
dc.date.accessioned | 2022-12-09T01:46:26Z | - |
dc.date.available | 2022-12-09T01:46:26Z | - |
dc.date.issued | 2022 | - |
dc.identifier.citation | Li, P., Jing, R., Zhou, M., Jia, P., Li, Z., Liu, G., Wang, Z. & Wang, H. (2022). Whole-cell biosynthesis of cytarabine by an unnecessary protein-reduced Escherichia coli that coexpresses purine and uracil phosphorylase. Biotechnology and Bioengineering, 119(7), 1768-1780. https://dx.doi.org/10.1002/bit.28098 | en_US |
dc.identifier.issn | 0006-3592 | en_US |
dc.identifier.uri | https://hdl.handle.net/10356/163555 | - |
dc.description.abstract | Currently, whole-cell catalysts face challenges due to the complexity of reaction systems, although they have a cost advantage over pure enzymes. In this study, cytarabine was synthesized by purified purine phosphorylase 1 (PNP1) and uracil phosphorylase (UP), and the conversion of cytarabine from adenine arabinoside reached 72.3 ± 4.3%. However, the synthesis was unsuccessful by whole-cell catalysis due to interference from unnecessary proteins (UNPs) in cells. Thus, we carried out a large-scale gene editing involving 377 genes in the genome of Escherichia coli to reduce the negative effect of UNPs on substrate conversion and cytarabine production. Finally, the PNP1 and UP activities of the obtained mutant were increased significantly compared with the parental strain, and more importantly, the conversion rate of cytarabine by whole-cell catalysis reached 67.4 ± 2.5%. The lack of 148 proteins and downregulation of 783 proteins caused by gene editing were equivalent to partial purification of the enzymes within cells, and thus, we provided inspiration to solve the problem caused by UNP interference, which is ubiquitous in the field of whole-cell catalysis. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Biotechnology and Bioengineering | en_US |
dc.rights | © 2022 Wiley Periodicals LLC. All rights reserved. | en_US |
dc.subject | Science::Biological sciences | en_US |
dc.title | Whole-cell biosynthesis of cytarabine by an unnecessary protein-reduced Escherichia coli that coexpresses purine and uracil phosphorylase | en_US |
dc.type | Journal Article | en |
dc.contributor.research | Nanyang Environment and Water Research Institute | en_US |
dc.contributor.research | Advanced Environmental Biotechnology Centre (AEBC) | en_US |
dc.identifier.doi | 10.1002/bit.28098 | - |
dc.identifier.pmid | 35383880 | - |
dc.identifier.scopus | 2-s2.0-85128072813 | - |
dc.identifier.issue | 7 | en_US |
dc.identifier.volume | 119 | en_US |
dc.identifier.spage | 1768 | en_US |
dc.identifier.epage | 1780 | en_US |
dc.subject.keywords | Cytarabine | en_US |
dc.subject.keywords | Escherichia Coli | en_US |
dc.description.acknowledgement | This study was supported by the Excellent Scientific and Technological Innovation Team of Henan Normal University, China (5101049170501) and the National Science Foundations of China (U160411067). | en_US |
item.fulltext | No Fulltext | - |
item.grantfulltext | none | - |
Appears in Collections: | NEWRI Journal Articles |
SCOPUSTM
Citations
50
2
Updated on Mar 23, 2024
Web of ScienceTM
Citations
50
2
Updated on Oct 30, 2023
Page view(s)
105
Updated on Mar 28, 2024
Google ScholarTM
Check
Altmetric
Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.