Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/163701
Full metadata record
DC FieldValueLanguage
dc.contributor.authorSingh, Hemang Rajen_US
dc.date.accessioned2022-12-15T13:18:06Z-
dc.date.available2022-12-15T13:18:06Z-
dc.date.issued2022-
dc.identifier.citationSingh, H. R. (2022). Towards precision dosing of nanoparticles in mammalian cells. Final Year Project (FYP), Nanyang Technological University, Singapore. https://hdl.handle.net/10356/163701en_US
dc.identifier.urihttps://hdl.handle.net/10356/163701-
dc.description.abstractCurrent in vitro assays of nanotoxicity studies rely on suspension-based delivery of ENMs into cells, which are in turn internalised by endocytosis. However, transformations of ENMs in suspension affect mass transport of particles from media to the cells, failure to characterise accurate dosages of NPs delivered into cells result in some of the disparity between in vitro and in vivo toxicity assays. This study explores alternative methods of intracellular delivery, relying on membrane-disruption, instead of endocytosis, to improve precision in delivery of ENMs into cells. Hydroporator is one such microfluidic intracellular delivery platform that hydrodynamically deforms cells, forming transient nanopores in plasma membrane through which particles can enter. Using carboxylic-functionalized polystyrene nanoparticles (COOH-PS NP) as model nanoparticulate system, our study found that while the hydroporator is able to achieve high-efficiency delivery (>94% of cells) of Polystyrene nanospheres into cells, it does put stresses onto the cells, inducing ROS generation and temporary cell-cycle stalling. However, it does not otherwise prompt COOH-PS, an otherwise non-toxic NP into toxicity. Further examinations also show that membrane-disruption based intake does not entirely prevent lysosomal capture but does allow more particles to evade such fate than in endocytosis-mediated uptake.en_US
dc.language.isoenen_US
dc.publisherNanyang Technological Universityen_US
dc.relationMSE/21/230en_US
dc.subjectEngineering::Nanotechnologyen_US
dc.subjectEngineering::Materials::Biomaterialsen_US
dc.titleTowards precision dosing of nanoparticles in mammalian cellsen_US
dc.typeFinal Year Project (FYP)en_US
dc.contributor.supervisorDalton Tay Chor Yongen_US
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.description.degreeBachelor of Engineering (Materials Engineering)en_US
dc.contributor.supervisoremailcytay@ntu.edu.sgen_US
item.fulltextWith Fulltext-
item.grantfulltextembargo_restricted_20241216-
Appears in Collections:MSE Student Reports (FYP/IA/PA/PI)
Files in This Item:
File Description SizeFormat 
FYP-REPORT_SinghHemangRaj - DT_3 copy.pdf
  Until 2024-12-16
1.69 MBAdobe PDFUnder embargo until Dec 16, 2024

Page view(s)

152
Updated on May 26, 2024

Google ScholarTM

Check

Items in DR-NTU are protected by copyright, with all rights reserved, unless otherwise indicated.