Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164052
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dc.contributor.authorXiong, Aolien_US
dc.contributor.authorKaushal, Simranen_US
dc.contributor.authorTay, Ian Junjieen_US
dc.contributor.authorEngelward, Bevin P.en_US
dc.contributor.authorHan, Jongyoonen_US
dc.contributor.authorPreiser, Peter Raineren_US
dc.date.accessioned2023-01-03T08:07:55Z-
dc.date.available2023-01-03T08:07:55Z-
dc.date.issued2021-
dc.identifier.citationXiong, A., Kaushal, S., Tay, I. J., Engelward, B. P., Han, J. & Preiser, P. R. (2021). MalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparum. STAR Protocols, 2(3), 100797-. https://dx.doi.org/10.1016/j.xpro.2021.100797en_US
dc.identifier.issn2666-1667en_US
dc.identifier.urihttps://hdl.handle.net/10356/164052-
dc.description.abstractComet assay is a standard approach for studying DNA damage in malaria, but high-throughput options are not available. The CometChip was previously developed using mammalian cells as a high-throughput version of the comet assay. It is based on the same principle as the comet assay but provides greater efficacy, automated data processing, and improved consistency between experiments. In this protocol, we present MalariaCometChip to quantitatively assess drug-induced DNA damage in Plasmodium falciparum. For complete details on the use and execution of this protocol, please refer to Xiong et al. (2020).en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.language.isoenen_US
dc.relation.ispartofSTAR Protocolsen_US
dc.rights© 2021 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).en_US
dc.subjectScience::Biological sciencesen_US
dc.titleMalariaCometChip for high-throughput quantification of DNA damage in Plasmodium falciparumen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.organizationBioSystems and Micromechanics (BioSyM) interdisciplinary research group (IRG), Singapore-MIT Alliance for Research and Technology (SMART)en_US
dc.contributor.organizationAnti-Microbial Resistance (AMR) IRG, Singapore-MIT Alliance for Research and Technology (SMART)en_US
dc.identifier.doi10.1016/j.xpro.2021.100797-
dc.description.versionPublished versionen_US
dc.identifier.pmid34527954-
dc.identifier.scopus2-s2.0-85122802034-
dc.identifier.issue3en_US
dc.identifier.volume2en_US
dc.identifier.spage100797en_US
dc.subject.keywordsHigh-Throughput Screeningen_US
dc.subject.keywordsComet Assayen_US
dc.description.acknowledgementThis work was supported by the National Research Foundation, Prime Minister’s Office, Singapore, through the Singapore-MIT Alliance for Research and Technology (SMART) BioSystems and Micromechanics (BioSym) Interdisciplinary Research Group (IRG) (to A.X. and J.H.) and Antimicrobial Resistance IRG (to A.X., J.H., and P.R.P.); by the Bill and Melinda Gates Foundation (Global Health grant number OPP1040463 to A.X. and P.R.P.); S.K. was supported by the National Institute of Environmental Health Sciences Superfund Basic Research Program (NIH P42 ES027707) to promote research translation.en_US
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