Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164053
Title: A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae
Authors: Ho, Nurulain
Yap, Wei Sheng
Thibault, Guillaume
Keywords: Science::Biological sciences
Issue Date: 2021
Source: Ho, N., Yap, W. S. & Thibault, G. (2021). A high-throughput genetic screening protocol to measure lipid bilayer stress-induced unfolded protein response in Saccharomyces cerevisiae. STAR Protocols, 2(4), 100868-. https://dx.doi.org/10.1016/j.xpro.2021.100868
Project: 2019-T1-002-011
NRF2018NRFNSFC003SB-006
Journal: STAR Protocols
Abstract: The endoplasmic reticulum (ER) stress is defined by the accumulation of unfolded proteins at the ER and perturbation at the ER membrane, known as lipid bilayer stress (LBS). In turn, ER stress triggers the unfolded protein response (UPR) to restore ER homeostasis. Here, we provide a modified protocol based on the synthetic genetic array analysis in Saccharomyces cerevisiae to identify genetic perturbations that induce the UPR by LBS. This method is adaptable to other canonical stress pathways. For complete details on the use and execution of this protocol, please refer to Ho et al. (2020), Jonikas et al. (2009) and Baryshnikova et al. (2010).
URI: https://hdl.handle.net/10356/164053
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100868
Schools: School of Biological Sciences 
Organisations: Institute of Molecular and Cell Biology, A*STAR
Rights: © 2021 The Authors. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Fulltext Permission: open
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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