Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164055
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dc.contributor.authorYao, Xuefengen_US
dc.contributor.authorLi, Huaqiongen_US
dc.contributor.authorChen, Lipingen_US
dc.contributor.authorTan, Lay Pohen_US
dc.date.accessioned2023-01-03T08:32:54Z-
dc.date.available2023-01-03T08:32:54Z-
dc.date.issued2022-
dc.identifier.citationYao, X., Li, H., Chen, L. & Tan, L. P. (2022). UV-induced senescence of human dermal fibroblasts restrained by low-stiffness matrix by inhibiting NF-κB activation. Engineered Regeneration, 3(4), 365-373. https://dx.doi.org/10.1016/j.engreg.2022.08.002en_US
dc.identifier.issn2666-1381en_US
dc.identifier.urihttps://hdl.handle.net/10356/164055-
dc.description.abstractAs a hallmark of skin aging, senescent human dermal fibroblasts (HDFs) are known to lose the ability to divide. However, they can still interact with their cellular environment and the surrounding matrix. As the skin ages, the progressive slowing down of HDFs function decreases the skin's structural integrity, which is more serious than if there is the dermal collagen matrix eroded. This leads to matters of the unbalanced barrier under the skin, skin fragility, inadequate wound healing, as well as other cosmetic issues. It is also well documented that skin aging comes with significant stiffness increases. Therefore, understanding the interactions between HDFs and the surrounding microenvironments during senescence may provide insights into skin aging. Here we aim to investigate matrix stiffness' effect on HDF senescence and elucidate possible mechanisms that make HDFs senescent. In our experiments, HDFs were cultivated on Polydimethylsiloxane (PDMS) with various stiffnesses and exposed to UV light to trigger senescence. Results show that HDFs are significantly affected by senescence when cultured on a matrix with stiffness. However, the cells are not significantly affected when cultured on a low stiffness matrix. The following characterization revealed cells cultured on stiff substrates under UV exposure had stimulated the nucleus factor kappa-B (NF-κB) activation. In contrast, cells on a matrix of softness only displayed low activation of NF-κB. NF-κB activity suppression with ammonium pyrrolidine dithiocarbamate (PDTC) decreases UV-induced HDFs senescence on stiff substrates. Taken together, we demonstrated that soft matrix defends HDFs against ultraviolet-induced senescence by inhibiting the activation of NF-κB.en_US
dc.description.sponsorshipNanyang Technological Universityen_US
dc.language.isoenen_US
dc.relation.ispartofEngineered Regenerationen_US
dc.subjectEngineering::Materialsen_US
dc.titleUV-induced senescence of human dermal fibroblasts restrained by low-stiffness matrix by inhibiting NF-κB activationen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Materials Science and Engineeringen_US
dc.identifier.doi10.1016/j.engreg.2022.08.002-
dc.identifier.scopus2-s2.0-85136133248-
dc.identifier.issue4en_US
dc.identifier.volume3en_US
dc.identifier.spage365en_US
dc.identifier.epage373en_US
dc.subject.keywordsHuman Dermal Fibroblastsen_US
dc.subject.keywordsMatrix Stiffnessen_US
dc.description.acknowledgementThe authors acknowledged the financial support from Nanyang Technological University, Singapore for PhD scholarship and funding; Zhejiang Provincial Natural Science Foundation (LY20C070010), and start-up funding from Wenzhou Institute, University of Chinese Academy of Sciences (WIUCASQD2019002).en_US
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