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Title: Studying the organization of the Golgi by super-resolution microscopy
Authors: Tie, Hieng Chiong
Lu, Lei
Keywords: Science::Biological sciences::Cytology
Issue Date: 2023
Source: Tie, H. C. & Lu, L. (2023). Studying the organization of the Golgi by super-resolution microscopy. Methods in Molecular Biology, 2557, 113-126.
Project: MOE2018-T2-2-026
Journal: Methods in Molecular Biology
Abstract: The Golgi complex is essential for protein transport and posttranslational modification in mammalian cells. It is critical to know the cisternal distribution of Golgi proteins to understand Golgi functions. The cis-to-trans or axial localization of a Golgi protein can be obtained using our previously developed method, Golgi protein localization by imaging centers of mass (GLIM), in nocodazole-induced Golgi ministacks (hereafter referred to as ministacks). However, there is no effective light microscopic method to reveal the lateral localization of a Golgi protein, which is the distribution within the Golgi cisternae. The challenge is partially caused by the random orientations and the tight congregation of Golgi stacks at the perinuclear region. Here, we summarize our method to identify en face and side views of ministacks. It takes advantage of the characteristic ring and double-punctum staining patterns exhibited by cisternal rim-localized proteins. After averaging multiple en face views, the resulting image reveals the intrinsic organization of cisternae in a non-biased manner.
ISBN: 978-1-0716-2638-2
ISSN: 1064-3745
DOI: 10.1007/978-1-0716-2639-9_9
Schools: School of Biological Sciences 
Rights: © 2023 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. All rights reserved. This version of the article has been accepted for publication, after peer review and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at:
Fulltext Permission: embargo_20241221
Fulltext Availability: With Fulltext
Appears in Collections:SBS Journal Articles

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