Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164113
Title: Protein-mediated fluorescence resonance energy transfer (P-FRET) probe: fabrication and hydroxyl radical detection
Authors: Yu, Xiaokan
Zhu, Weisheng
Ouyang, Wenao
Zhang, Xiaojia
Qiu, Hao
Zhang, Zhijun
Xing, Bengang
Keywords: Science::Chemistry
Issue Date: 2022
Source: Yu, X., Zhu, W., Ouyang, W., Zhang, X., Qiu, H., Zhang, Z. & Xing, B. (2022). Protein-mediated fluorescence resonance energy transfer (P-FRET) probe: fabrication and hydroxyl radical detection. Photochemistry and Photobiology, 98(2), 371-377. https://dx.doi.org/10.1111/php.13595
Project: RG6/20
A1983c0028 (M4070319)
A20E5c0090
Journal: Photochemistry and Photobiology
Abstract: Fluorescent probes based on fluorescence resonance energy transfer (FRET) are highly promising for diverse bioapplications. The key to constructing FRET probes is to confine the donor and acceptor within a sufficiently close distance. However, the commonly used covalent linkage often requires elaborate design and complex organic synthesis, and sometimes causes changes in the fluorescence properties of the donor and acceptor. Inspired by the binding between small molecules and protein in nature, herein, we propose a protein-mediated strategy to fabricate FRET probe. In such protein-mediated FRET (P-FRET) probe, protein acts as a carrier to simultaneously confine donor and acceptor in its cavity. As a proof of concept, we use bovine serum albumin (BSA) as a model protein, coumarin derivative as a donor and hydroxyl radical (·OH)-responsive dye fluorescein as an acceptor. Through a series of investigations, including binding parameters, fluorescence properties and detection performance, we prove that the construction of P-FRET probe is simple and feasible and the detection is sensitive. Our P-FRET strategy will provide new insights for the design of FRET probes.
URI: https://hdl.handle.net/10356/164113
ISSN: 0031-8655
DOI: 10.1111/php.13595
Rights: © 2022 American Society for Photobiology. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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