Please use this identifier to cite or link to this item: https://hdl.handle.net/10356/164118
Title: Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS)
Authors: Armstrong, Michael D. Sorochan
Campos, O. René Arredondo
Bannon, Catherine C.
de la Mata, A. Paulina
Case, Rebecca J.
Harynuk, James J.
Keywords: Science::Biological sciences
Issue Date: 2022
Source: Armstrong, M. D. S., Campos, O. R. A., Bannon, C. C., de la Mata, A. P., Case, R. J. & Harynuk, J. J. (2022). Global metabolome analysis of Dunaliella tertiolecta, Phaeobacter italicus R11 co-cultures using thermal desorption - comprehensive two-dimensional gas chromatography - time-of-flight mass spectrometry (TD-GC×GC-TOFMS). Phytochemistry, 195, 113052-. https://dx.doi.org/10.1016/j.phytochem.2021.113052
Project: A20H7a0152
Journal: Phytochemistry
Abstract: Dunaliella tertiolecta is a marine microalgae that has been studied extensively as a potential carbon-neutral biofuel source (Tang et al., 2011). Microalgae oil contains high quantities of energy-rich fatty acids and lipids, but is not yet commercially viable as an alternative fuel. Carefully optimised growth conditions, and more recently, algal-bacterial co-cultures have been explored as a way of improving the yield of D. tertiolecta microalgae oils. The relationship between the host microalgae and bacterial co-cultures is currently poorly understood. Here, a complete workflow is proposed to analyse the global metabolomic profile of co-cultured D. tertiolectra and Phaeobacter italicus R11, which will enable researchers to explore the chemical nature of this relationship in more detail. To the best of the authors' knowledge this study is one of the first of its kind, in which a pipeline for an entirely untargeted analysis of the algal metabolome is proposed using a practical sample preparation, introduction, and data analysis routine.
URI: https://hdl.handle.net/10356/164118
ISSN: 0031-9422
DOI: 10.1016/j.phytochem.2021.113052
Schools: School of Biological Sciences 
Research Centres: Singapore Centre for Environmental Life Sciences and Engineering 
Rights: © 2021 Elsevier Ltd. All rights reserved.
Fulltext Permission: none
Fulltext Availability: No Fulltext
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