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Title: Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants
Authors: Lim, Sing Mei
Cheng, Hoi Lok
Jia, Huan
Kongsuphol, Patthara
Bhuvaneshwari D/O Shunmuganathan
Chen, Ming Wei
Ng, Say Yong
Gao, Xiaohong
Turaga, Shuvan Prashant
Heussler, Sascha P.
Somani, Jyoti
Sengupta, Sharmila
Tay, Dousabel M. Y.
McBee, Megan E.
Young, Barnaby Edward
MacAry, Paul A.
Sikes, Hadley D.
Preiser, Peter Rainer
Keywords: Science::Medicine
Issue Date: 2022
Source: Lim, S. M., Cheng, H. L., Jia, H., Kongsuphol, P., Bhuvaneshwari D/O Shunmuganathan, Chen, M. W., Ng, S. Y., Gao, X., Turaga, S. P., Heussler, S. P., Somani, J., Sengupta, S., Tay, D. M. Y., McBee, M. E., Young, B. E., MacAry, P. A., Sikes, H. D. & Preiser, P. R. (2022). Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants. Bioengineering and Translational Medicine, 7(2), e10293-.
Project: NHIC-COVID19-2005004 
Journal: Bioengineering and Translational Medicine 
Abstract: There is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.
ISSN: 2380-6761
DOI: 10.1002/btm2.10293
Schools: School of Biological Sciences 
Lee Kong Chian School of Medicine (LKCMedicine) 
Organisations: Singapore-MIT Alliance in Research and Technology (SMART)
National Centre for Infectious Diseases
Tan Tock Seng Hospital
Rights: © 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Fulltext Permission: open
Fulltext Availability: With Fulltext
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