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dc.contributor.authorLim, Sing Meien_US
dc.contributor.authorCheng, Hoi Loken_US
dc.contributor.authorJia, Huanen_US
dc.contributor.authorKongsuphol, Pattharaen_US
dc.contributor.authorBhuvaneshwari D/O Shunmuganathanen_US
dc.contributor.authorChen, Ming Weien_US
dc.contributor.authorNg, Say Yongen_US
dc.contributor.authorGao, Xiaohongen_US
dc.contributor.authorTuraga, Shuvan Prashanten_US
dc.contributor.authorHeussler, Sascha P.en_US
dc.contributor.authorSomani, Jyotien_US
dc.contributor.authorSengupta, Sharmilaen_US
dc.contributor.authorTay, Dousabel M. Y.en_US
dc.contributor.authorMcBee, Megan E.en_US
dc.contributor.authorYoung, Barnaby Edwarden_US
dc.contributor.authorMacAry, Paul A.en_US
dc.contributor.authorSikes, Hadley D.en_US
dc.contributor.authorPreiser, Peter Raineren_US
dc.identifier.citationLim, S. M., Cheng, H. L., Jia, H., Kongsuphol, P., Bhuvaneshwari D/O Shunmuganathan, Chen, M. W., Ng, S. Y., Gao, X., Turaga, S. P., Heussler, S. P., Somani, J., Sengupta, S., Tay, D. M. Y., McBee, M. E., Young, B. E., MacAry, P. A., Sikes, H. D. & Preiser, P. R. (2022). Finger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variants. Bioengineering and Translational Medicine, 7(2), e10293-.
dc.description.abstractThere is clinical need for a quantifiable point-of-care (PoC) SARS-CoV-2 neutralizing antibody (nAb) test that is adaptable with the pandemic's changing landscape. Here, we present a rapid and semi-quantitative nAb test that uses finger stick or venous blood to assess the nAb response of vaccinated population against wild-type (WT), alpha, beta, gamma, and delta variant RBDs. It captures a clinically relevant range of nAb levels, and effectively differentiates prevaccination, post first dose, and post second dose vaccination samples within 10 min. The data observed against alpha, beta, gamma, and delta variants agrees with published results evaluated in established serology tests. Finally, our test revealed a substantial reduction in nAb level for beta, gamma, and delta variants between early BNT162b2 vaccination group (within 3 months) and later vaccination group (post 3 months). This test is highly suited for PoC settings and provides an insightful nAb response in a postvaccinated population.en_US
dc.description.sponsorshipMinistry of Health (MOH)en_US
dc.description.sponsorshipNational Medical Research Council (NMRC)en_US
dc.description.sponsorshipNational Research Foundation (NRF)en_US
dc.relation.ispartofBioengineering and Translational Medicineen_US
dc.rights© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en_US
dc.titleFinger stick blood test to assess postvaccination SARS-CoV-2 neutralizing antibody response against variantsen_US
dc.typeJournal Articleen
dc.contributor.schoolSchool of Biological Sciencesen_US
dc.contributor.schoolLee Kong Chian School of Medicine (LKCMedicine)en_US
dc.contributor.organizationSingapore-MIT Alliance in Research and Technology (SMART)en_US
dc.contributor.organizationNational Centre for Infectious Diseasesen_US
dc.contributor.organizationTan Tock Seng Hospitalen_US
dc.description.versionPublished versionen_US
dc.subject.keywordsCellulose Pulldown Assayen_US
dc.description.acknowledgementThis study is supported by National Health Innovation Singapore (NHIC) grant # NHIC-COVID19-2005004, National Research Foundation via CREATE Share grant #R571-002-021-592 and the Anti-microbial Resistance Interdisciplinary Research Group (AMR-IRG) of Singapore-MIT Alliance in Research and Technology (SMART). All samples acquired from National Centre for Infectious Diseases (NCID) were supported by Singapore Ministry of Health's National Medical Research Council COVID-19 Research Fund: COVID19RF-0008.en_US
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