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dc.contributor.authorNgo, Khac Huyen_US
dc.identifier.citationNgo, K. H. (2022). Structure and protein recognition of G-quadruplexes. Doctoral thesis, Nanyang Technological University, Singapore.
dc.description.abstractG-rich oligonucleotides can adopt non-canonical secondary structures called G-quadruplexes (G4s) which are biologically relevant and found in applications and nanotechnology. Due to the high structural polymorphism, it is still a challenge to specifically target different G4 structures. In this study, different biophysical, biochemical, and structural techniques (CD, ITC, gel electrophoresis, enzymatic assay NMR spectroscopy, X-ray crystallography) and nucleic acid/protein/peptide engineering were applied to: i) expand our understanding of G4 diversity including base stacking, interlocking, and A•G•G•G•G pentad formation; ii) study the molecular basis for G4 recognition by proteins; iii) engineer G4-binding proteins/peptides for various applications including stable cyclic peptide or engineered enzyme for G4s targeting. The findings from this study provide a better understanding of G4 structures and their recognition by proteins.en_US
dc.publisherNanyang Technological Universityen_US
dc.rightsThis work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0).en_US
dc.titleStructure and protein recognition of G-quadruplexesen_US
dc.typeThesis-Doctor of Philosophyen_US
dc.contributor.supervisorPhan Anh Tuanen_US
dc.contributor.schoolSchool of Physical and Mathematical Sciencesen_US
dc.description.degreeDoctor of Philosophyen_US
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